Q8YJ71 (RPPH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA pyrophosphohydrolase
EC=3.6.1.-

Alternative name(s):
(Di)nucleoside polyphosphate hydrolase

Gene names

Name:	rppH
Synonyms:	nudH
Ordered Locus Names:	BMEI0215

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage By similarity. HAMAP MF_00298
Cofactor	Divalent ions By similarity. HAMAP MF_00298
Sequence similarities	Belongs to the Nudix hydrolase family. RppH subfamily. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	RNA pyrophosphohydrolase HAMAP MF_00298		PRO_0000056998
Regions
	Domain	18 – 171	154	Nudix hydrolase
	Motif	59 – 80	22	Nudix box HAMAP MF_00298

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ71 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1658DE70E87C933B	FASTA	178	19,855
	10 20 30 40 50 60 MSEHKGPTGA MVDPESLPYR PCVGLMVLNK AGLVWAGRRI VIPGDEMDGA TQLWQMPQGG 70 80 90 100 110 120 IDKGEDPAQA ALRELYEETG MTSVSLLEEA SDWINYDLPP HLVGLALKGK YRGQTQKWFA 130 140 150 160 170 YRFEGDESEI AINPPPGGHT AEFDCWEWKP MADLPNLIVP FKRKVYEQVV ATFRHLAA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51397.1.
PIR	AB3279.
RefSeq	NP_539133.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ71.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195927.
GenomeReviews	Gene locus BMEI0215 in contig AE008917_GR.
KEGG	bme:BMEI0215.
NMPDR	fig|224914.1.peg.215.
PATRIC	17802042. VBIBruMel146950_3338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302451.
OMA	HEGKVWV.
PhylomeDB	Q8YJ71.
ProtClustDB	PRK00714.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0215-MONOMER.
Family and domain databases
HAMAP	MF_00298. Nudix_RppH. [Tree]
InterPro	IPR020476. Nudix_hydrolase. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. IPR022927. RppH. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
KO	K08311.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR00502. NUDIXFAMILY.
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPPH_BRUME

Accession

Primary (citable) accession number: Q8YJ71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program