Q8YJ50 (HTPX_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease HtpX homolog
EC=3.4.24.-

Gene names

Name:	htpX
Ordered Locus Names:	BMEI0236

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00188
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00188.
Sequence similarities	Belongs to the peptidase M48B family.
Sequence caution	The sequence AAL51418.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Protease HtpX homolog HAMAP-Rule MF_00188		PRO_0000138854
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
Sites
	Active site	131	1	By similarity
	Metal binding	130	1	Zinc; catalytic By similarity
	Metal binding	134	1	Zinc; catalytic By similarity
	Metal binding	202	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ50 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: 92F2DBFD8D8B4D13	FASTA	325	34,472
	10 20 30 40 50 60 MNMTKTAMLI ALMTVMFMSI GYLLGGGGGM MIALVIAVAM NLFGYWNSDK MVLRMYNAQE 70 80 90 100 110 120 VDERSAPEYY RMVSGLAANA GLPMPKVYII HEDQPNAFAT GRNPENAAVA ATTGLLNWLS 130 140 150 160 170 180 PEEVAGVMAH ELAHVQNRDT LTMTIVATLA GAISMLGNFA FFLGGNRENG NGVMGVVGTL 190 200 210 220 230 240 LAMIVAPFGA MIVQMAVSRT REYAADKRGA EICGNPLWLS SALGKIARGA KVIPNEEAEH 250 260 270 280 290 300 NPATAHMFII NPLSGRGADN LFSTHPDTDN RIAALEQMAA ETGIRSAAMT ARAAAPSQNS 310 320 GPWGQRSDNA GGNSNGGSRY RGPWS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51418.1. Different initiation.
PIR	AG3281.
RefSeq	NP_539154.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ50.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0236.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51418; AAL51418; BMEI0236.
GeneID	1195948.
KEGG	bme:BMEI0236.
PATRIC	17850470. VBIBruMel92729_0258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000227301.
KO	K03799.
OMA	YGYWNSD.
ProtClustDB	PRK01345.
Enzyme and pathway databases
BioCyc	BMEL224914:GCJ0-247-MONOMER.
Family and domain databases
HAMAP	MF_00188. Peptidase_M48.
InterPro	IPR022919. Pept_M48_protease_HtpX. IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTPX_BRUME

Accession

Primary (citable) accession number: Q8YJ50

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	May 29, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program