Reviewed, UniProtKB/Swiss-Prot Q8YJ50 (HTPX_BRUME)

Names and origin

Protein names

Recommended name:
    Probable protease htpX homolog
    EC=3.4.24.-

Gene names

Name:	htpX
Ordered Locus Names:	BMEI0236

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase M48B family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Probable protease htpX homolog HAMAP MF_00188		PRO_0000138854
Regions
	Transmembrane	7 – 24	18	Potential
	Transmembrane	29 – 46	18	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	173 – 193	21	Potential
Sites
	Active site	131	1	By similarity
	Metal binding	130	1	Zinc; catalytic By similarity
	Metal binding	134	1	Zinc; catalytic By similarity
	Metal binding	202	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ50-1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: 92F2DBFD8D8B4D13	FASTA	325	34,472
	10 20 30 40 50 60 MNMTKTAMLI ALMTVMFMSI GYLLGGGGGM MIALVIAVAM NLFGYWNSDK MVLRMYNAQE 70 80 90 100 110 120 VDERSAPEYY RMVSGLAANA GLPMPKVYII HEDQPNAFAT GRNPENAAVA ATTGLLNWLS 130 140 150 160 170 180 PEEVAGVMAH ELAHVQNRDT LTMTIVATLA GAISMLGNFA FFLGGNRENG NGVMGVVGTL 190 200 210 220 230 240 LAMIVAPFGA MIVQMAVSRT REYAADKRGA EICGNPLWLS SALGKIARGA KVIPNEEAEH 250 260 270 280 290 300 NPATAHMFII NPLSGRGADN LFSTHPDTDN RIAALEQMAA ETGIRSAAMT ARAAAPSQNS 310 320 GPWGQRSDNA GGNSNGGSRY RGPWS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51418.1. Different initiation.
PIR	AG3281.
RefSeq	NP_539154.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M48.004.
Genome annotation databases
GeneID	1195948.
GenomeReviews	Gene locus BMEI0236 in contig AE008917_GR.
KEGG	bme:BMEI0236.
NMPDR	fig|224914.1.peg.236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG739460.
OMA	NAAHQVP.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0236-MON.
Family and domain databases
HAMAP	MF_00188. [Tree]
InterPro	IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTPX_BRUME

Accession

Primary (citable) accession number: Q8YJ50

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	December 15, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)