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Q8YJ44

- SYL_BRUME

UniProt

Q8YJ44 - SYL_BRUME

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Protein
Leucine--tRNA ligase
Gene
leuS, BMEI0242
Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:BMEI0242
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Leucine--tRNA ligaseUniRule annotation
PRO_0000151985Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI0242.

Structurei

3D structure databases

ProteinModelPortaliQ8YJ44.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionUniRule annotation
Add
BLAST
Motifi628 – 6325"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000200747.
KOiK01869.
OMAiPVIHCPK.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YJ44-1 [UniParc]FASTAAdd to Basket

« Hide

MAAERYNPRV AEAHWQKVWE ENRTFETDNS DSREKYYVLE MFPYPSGRIH    50
MGHVRNYAMG DVVARYKRAK GFNVLHPMGW DAFGMPAENA AMQNKVHPKE 100
WTYQNIATMK RQLKSMGLSL DWSREFATCD VEYYHRQQML FIDLYEKGLV 150
TRKTSKVNWD PVDNTVLANE QVVDGRGWRS GALVEQRELT QWFFKITDFS 200
EELLAGLDTL DQWPEKVRLM QRNWIGKSEG LQVRFALAAG TAPAGFSEVE 250
VYTTRPDTLF GAAFVAISAD HPLAKKLSEG NAALSSFIEE CHQQGTSLAA 300
LETAEKKGFD TGIKVKHPFD DNWELPVYVA NFVLMEYGTG AVFGCPAHDQ 350
RDLDFANKYK LKVTPVVLPK GEDAASFSIG ETAYTDDGVM INSRFLDGMT 400
PEAAFNEVAS RLEKTDLVGR PQAVRKVQFR LRDWGISRQR YWGCPIPMIH 450
CESCGVNPVP RADLPVKLPD DVEFDRPGNP LDRHATWRHV KCPKCGGDAR 500
RETDTMDTFV DSSWYYTRFT APWENEPTDR KAADHWLPVD QYIGGIEHAI 550
LHLLYSRFFT RAMKVAGHVG VDEPFKGLFT QGMVVHETYK ANGQWVSPAD 600
IRIEEIDGKR VATMLDSGAP VEIGSIEKMS KSKKNVVDPD DIIASYGADT 650
ARWFVLSDSP PERDVIWTEA GAEGAHRFVQ RIWRLVAEAA PALKDVAPKA 700
GTQGEALGVS KAAHKAVKAV GDDIEKLAFN RGVARLYELV NTLSGALQQA 750
ADGKADAEMK GALREATEML VLMTAPMMPH LAEQCLAELG GKVAGKETLV 800
ARAPWPVFDP ALVVENEIVL PVQINGKKRG DLTIARDADQ ASIQQAVLEL 850
DFVKAALNGG SPKKIIVVPQ RIVNVVA 877
Length:877
Mass (Da):97,863
Last modified:March 1, 2002 - v1
Checksum:i56D2AAA6E29FFD95
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51424.1.
PIRiAE3282.
RefSeqiNP_539160.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51424; AAL51424; BMEI0242.
GeneIDi1195954.
KEGGibme:BMEI0242.
PATRICi17802106. VBIBruMel146950_3370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51424.1 .
PIRi AE3282.
RefSeqi NP_539160.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YJ44.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0242.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL51424 ; AAL51424 ; BMEI0242 .
GeneIDi 1195954.
KEGGi bme:BMEI0242.
PATRICi 17802106. VBIBruMel146950_3370.

Phylogenomic databases

HOGENOMi HOG000200747.
KOi K01869.
OMAi PVIHCPK.
OrthoDBi EOG63Z74X.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiSYL_BRUME
AccessioniPrimary (citable) accession number: Q8YJ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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