Reviewed, UniProtKB/Swiss-Prot Q8YJ29 (HYPRE_BRUME)

Names and origin

Protein names

Recommended name:
    4-hydroxyproline epimerase
    EC=5.1.1.8
Alternative name(s):
    Hydroxyproline-2-epimerase
      Short name=BmHyPRE

Gene names

Ordered Locus Names:

BMEI0257

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.
Catalytic activity	Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline. Ref.1
Enzyme regulation	Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM. Ref.1
Subunit structure	Homodimer By similarity.
Miscellaneous	This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.
Sequence similarities	Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.
Biophysicochemical properties	Kinetic parameters: KM=9.0 mM for 4-hydroxy-L-proline KM=12.7 mM for 4-hydroxy-D-proline Vmax=0.45 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius) Vmax=0.70 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4-hydroxyproline epimerase activity Inferred from electronic annotation. Source: EC proline racemase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	4-hydroxyproline epimerase		PRO_0000354029
Sites
	Active site	90	1	Proton acceptor By similarity
	Active site	253	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ29-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 76457EF17BA72653	FASTA	333	36,511
	10 20 30 40 50 60 MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKCAHF LAEYDWIRTG LMFEPRGHDM 70 80 90 100 110 120 MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM AIEQGLVTPK TPGKLNLDTP 130 140 150 160 170 180 AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE GLEVECPDLG PIKVDVAYGG NFYAIVEPQE 190 200 210 220 230 240 NYTDMDDYSA LQLIAWSPVL RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA 250 260 270 280 290 300 VFYGDKAIDR SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP 310 320 330 AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens." Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P. PLoS ONE 2:E885-E885(2007) [PubMed: 17849014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF495342 Genomic DNA. Translation: ABS82394.1. AE008917 Genomic DNA. Translation: AAL51439.1.
PIR	AD3284.
RefSeq	NP_539175.1.
3D structure databases
HSSP	HSSP built from PDB template 1W61 based on UniProtKB Q4DA80.
ModBase	Search...
Genome annotation databases
GeneID	1195969.
GenomeReviews	Gene locus BMEI0257 in contig AE008917_GR.
KEGG	bme:BMEI0257.
NMPDR	fig|224914.1.peg.257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJ29.
OMA	EYDWIRK
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0257-MON.
Family and domain databases
InterPro	IPR008794. Pro_racemase. [Graphical view]
Pfam	PF05544. Pro_racemase. 1 hit. [Graphical view]
PIRSF	PIRSF029792. Pro_racemase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HYPRE_BRUME

Accession

Primary (citable) accession number: Q8YJ29

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)