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Protein

4-hydroxyproline epimerase

Gene

BMEI0257

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=9.0 mM for 4-hydroxy-L-proline1 Publication
  2. KM=12.7 mM for 4-hydroxy-D-proline1 Publication
  1. Vmax=0.45 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)1 Publication
  2. Vmax=0.70 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptorBy similarity
Binding sitei249 – 2491SubstrateBy similarity
Active sitei253 – 2531Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.1.1.8. 995.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyproline epimerase (EC:5.1.1.8)
Alternative name(s):
Hydroxyproline-2-epimerase
Short name:
BmHyPRE
Gene namesi
Ordered Locus Names:BMEI0257
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3333334-hydroxyproline epimerasePRO_0000354029Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224914.BAWG_2931.

Structurei

3D structure databases

ProteinModelPortaliQ8YJ29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 922Substrate bindingBy similarity
Regioni254 – 2552Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the proline racemase family.Curated

Phylogenomic databases

HOGENOMiHOG000084336.
KOiK12658.
OMAiCPCGLVT.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YJ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKCAHF LAEYDWIRTG
60 70 80 90 100
LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM
110 120 130 140 150
AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE
160 170 180 190 200
GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL
210 220 230 240 250
RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR
260 270 280 290 300
SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP
310 320 330
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SAA
Length:333
Mass (Da):36,511
Last modified:March 1, 2002 - v1
Checksum:i76457EF17BA72653
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF495342 Genomic DNA. Translation: ABS82394.1.
AE008917 Genomic DNA. Translation: AAL51439.1.
PIRiAD3284.
RefSeqiNP_539175.1. NC_003317.1.
WP_004684253.1. NZ_GG703781.1.

Genome annotation databases

EnsemblBacteriaiAAL51439; AAL51439; BMEI0257.
KEGGibme:BMEI0257.
PATRICi17802136. VBIBruMel146950_3385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF495342 Genomic DNA. Translation: ABS82394.1.
AE008917 Genomic DNA. Translation: AAL51439.1.
PIRiAD3284.
RefSeqiNP_539175.1. NC_003317.1.
WP_004684253.1. NZ_GG703781.1.

3D structure databases

ProteinModelPortaliQ8YJ29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_2931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51439; AAL51439; BMEI0257.
KEGGibme:BMEI0257.
PATRICi17802136. VBIBruMel146950_3385.

Phylogenomic databases

HOGENOMiHOG000084336.
KOiK12658.
OMAiCPCGLVT.
OrthoDBiEOG6SNDV3.

Enzyme and pathway databases

BRENDAi5.1.1.8. 995.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: 16M / ATCC 23456 / NCTC 10094.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry namei4HYPE_BRUME
AccessioniPrimary (citable) accession number: Q8YJ29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.