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Q8YJ29

- HYPRE_BRUME

UniProt

Q8YJ29 - HYPRE_BRUME

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Protein

4-hydroxyproline epimerase

Gene

BMEI0257

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=9.0 mM for 4-hydroxy-L-proline1 Publication
  2. KM=12.7 mM for 4-hydroxy-D-proline1 Publication

Vmax=0.45 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)1 Publication

Vmax=0.70 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptorBy similarity
Active sitei253 – 2531Proton donorBy similarity

GO - Molecular functioni

  1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
  2. proline racemase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.1.1.8. 995.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyproline epimerase (EC:5.1.1.8)
Alternative name(s):
Hydroxyproline-2-epimerase
Short name:
BmHyPRE
Gene namesi
Ordered Locus Names:BMEI0257
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3333334-hydroxyproline epimerasePRO_0000354029Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224914.BMEI0257.

Structurei

3D structure databases

ProteinModelPortaliQ8YJ29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000084336.
KOiK12658.
OMAiMIVDATI.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YJ29 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKCAHF LAEYDWIRTG
60 70 80 90 100
LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM
110 120 130 140 150
AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE
160 170 180 190 200
GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL
210 220 230 240 250
RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR
260 270 280 290 300
SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP
310 320 330
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SAA
Length:333
Mass (Da):36,511
Last modified:March 1, 2002 - v1
Checksum:i76457EF17BA72653
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495342 Genomic DNA. Translation: ABS82394.1.
AE008917 Genomic DNA. Translation: AAL51439.1.
PIRiAD3284.
RefSeqiNP_539175.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51439; AAL51439; BMEI0257.
GeneIDi1195969.
KEGGibme:BMEI0257.
PATRICi17802136. VBIBruMel146950_3385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495342 Genomic DNA. Translation: ABS82394.1 .
AE008917 Genomic DNA. Translation: AAL51439.1 .
PIRi AD3284.
RefSeqi NP_539175.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YJ29.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0257.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL51439 ; AAL51439 ; BMEI0257 .
GeneIDi 1195969.
KEGGi bme:BMEI0257.
PATRICi 17802136. VBIBruMel146950_3385.

Phylogenomic databases

HOGENOMi HOG000084336.
KOi K12658.
OMAi MIVDATI.
OrthoDBi EOG6SNDV3.

Enzyme and pathway databases

BRENDAi 5.1.1.8. 995.

Family and domain databases

InterProi IPR008794. Pro_racemase_fam.
[Graphical view ]
Pfami PF05544. Pro_racemase. 1 hit.
[Graphical view ]
PIRSFi PIRSF029792. Pro_racemase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: 16M / ATCC 23456 / NCTC 10094.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiHYPRE_BRUME
AccessioniPrimary (citable) accession number: Q8YJ29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 1, 2002
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3