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Q8YJ29 (HYPRE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyproline epimerase

EC=5.1.1.8
Alternative name(s):
Hydroxyproline-2-epimerase
Short name=BmHyPRE
Gene names
Ordered Locus Names:BMEI0257
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.

Catalytic activity

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline. Ref.1

Enzyme regulation

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM. Ref.1

Subunit structure

Homodimer By similarity.

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Sequence similarities

Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=9.0 mM for 4-hydroxy-L-proline Ref.1

KM=12.7 mM for 4-hydroxy-D-proline

Vmax=0.45 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)

Vmax=0.70 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Ontologies

Keywords
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_function4-hydroxyproline epimerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

proline racemase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3333334-hydroxyproline epimerase
PRO_0000354029

Sites

Active site901Proton acceptor By similarity
Active site2531Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YJ29 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 76457EF17BA72653

FASTA33336,511
        10         20         30         40         50         60 
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKCAHF LAEYDWIRTG LMFEPRGHDM 

        70         80         90        100        110        120 
MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM AIEQGLVTPK TPGKLNLDTP 

       130        140        150        160        170        180 
AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE GLEVECPDLG PIKVDVAYGG NFYAIVEPQE 

       190        200        210        220        230        240 
NYTDMDDYSA LQLIAWSPVL RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA 

       250        260        270        280        290        300 
VFYGDKAIDR SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP 

       310        320        330 
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SAA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: 16M / ATCC 23456 / NCTC 10094.
[2]"The genome sequence of the facultative intracellular pathogen Brucella melitensis."
DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. expand/collapse author list , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R.
Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF495342 Genomic DNA. Translation: ABS82394.1.
AE008917 Genomic DNA. Translation: AAL51439.1.
PIRAD3284.
RefSeqNP_539175.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YJ29.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0257.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51439; AAL51439; BMEI0257.
GeneID1195969.
KEGGbme:BMEI0257.
PATRIC17802136. VBIBruMel146950_3385.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000084336.
KOK12658.
OMAMIVDATI.
OrthoDBEOG6SNDV3.

Enzyme and pathway databases

BRENDA5.1.1.8. 995.

Family and domain databases

InterProIPR008794. Pro_racemase_fam.
[Graphical view]
PfamPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFPIRSF029792. Pro_racemase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHYPRE_BRUME
AccessionPrimary (citable) accession number: Q8YJ29
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names