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Q8YJ29

- HYPRE_BRUME

UniProt

Q8YJ29 - HYPRE_BRUME

Protein

4-hydroxyproline epimerase

Gene

BMEI0257

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.

    Catalytic activityi

    Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

    Enzyme regulationi

    Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

    Kineticsi

    1. KM=9.0 mM for 4-hydroxy-L-proline1 Publication
    2. KM=12.7 mM for 4-hydroxy-D-proline1 Publication

    Vmax=0.45 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)1 Publication

    Vmax=0.70 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Proton acceptorBy similarity
    Active sitei253 – 2531Proton donorBy similarity

    GO - Molecular functioni

    1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
    2. proline racemase activity Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BRENDAi5.1.1.8. 995.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyproline epimerase (EC:5.1.1.8)
    Alternative name(s):
    Hydroxyproline-2-epimerase
    Short name:
    BmHyPRE
    Gene namesi
    Ordered Locus Names:BMEI0257
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3333334-hydroxyproline epimerasePRO_0000354029Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi224914.BMEI0257.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YJ29.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000084336.
    KOiK12658.
    OMAiMIVDATI.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8YJ29-1 [UniParc]FASTAAdd to Basket

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    MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKCAHF LAEYDWIRTG    50
    LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM 100
    AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE 150
    GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL 200
    RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR 250
    SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP 300
    AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SAA 333
    Length:333
    Mass (Da):36,511
    Last modified:March 1, 2002 - v1
    Checksum:i76457EF17BA72653
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495342 Genomic DNA. Translation: ABS82394.1.
    AE008917 Genomic DNA. Translation: AAL51439.1.
    PIRiAD3284.
    RefSeqiNP_539175.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL51439; AAL51439; BMEI0257.
    GeneIDi1195969.
    KEGGibme:BMEI0257.
    PATRICi17802136. VBIBruMel146950_3385.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495342 Genomic DNA. Translation: ABS82394.1 .
    AE008917 Genomic DNA. Translation: AAL51439.1 .
    PIRi AD3284.
    RefSeqi NP_539175.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YJ29.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0257.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL51439 ; AAL51439 ; BMEI0257 .
    GeneIDi 1195969.
    KEGGi bme:BMEI0257.
    PATRICi 17802136. VBIBruMel146950_3385.

    Phylogenomic databases

    HOGENOMi HOG000084336.
    KOi K12658.
    OMAi MIVDATI.
    OrthoDBi EOG6SNDV3.

    Enzyme and pathway databases

    BRENDAi 5.1.1.8. 995.

    Family and domain databases

    InterProi IPR008794. Pro_racemase_fam.
    [Graphical view ]
    Pfami PF05544. Pro_racemase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF029792. Pro_racemase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
      Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
      PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: 16M / ATCC 23456 / NCTC 10094.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiHYPRE_BRUME
    AccessioniPrimary (citable) accession number: Q8YJ29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3