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Protein

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)

Gene

ispG

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.UniRule annotation

Catalytic activityi

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 5 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi307Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi310Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi353Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi360Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00096.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)UniRule annotation (EC:1.17.7.3UniRule annotation)
Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthaseUniRule annotation
Gene namesi
Name:ispGUniRule annotation
Ordered Locus Names:BMEI0269
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001905471 – 4204-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)Add BLAST420

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_2943.

Structurei

3D structure databases

ProteinModelPortaliQ8YJ17.
SMRiQ8YJ17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4E. Bacteria.
COG0821. LUCA.
HOGENOMiHOG000261017.
KOiK03526.
OMAiDVHHNGM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
PfamiView protein in Pfam
PF04551. GcpE. 1 hit.
PIRSFiPIRSF004640. IspG. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YJ17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSETVSYFS HPFPRRQSVG VSVGGVIVGG SAPVVVQSMT NTDTADVDST
60 70 80 90 100
VAQVAALHRA GSEIVRITVD RDESAAAVPK IRERLERLGH DVPLVGDFHY
110 120 130 140 150
IGHKLLADHP ACAEALAKYR INPGNVGFKD KKDKQFADIV EMAIRYDKPV
160 170 180 190 200
RIGVNWGSLD QELLTTLMDR NQAEGAPLSA QDVMREAIVQ SALISANLAE
210 220 230 240 250
EIGLGRDKII LSAKVSQVQD LIAVYTMLAQ RSNHALHLGL TEAGMGTKGI
260 270 280 290 300
VASSAAMGIL LQQGIGDTIR ISLTPEPGGD RTREVQVAQE LLQTMGFRQF
310 320 330 340 350
VPIVAACPGC GRTTSTVFQE LAQTIQEDIR RNMPLWREKY PGVEALSVAV
360 370 380 390 400
MGCIVNGPGE SKHADIGISL PGTGETPSAP VFVDGKKVTT LRGPGIAEDF
410 420
QKMVADYIEN RFGLGRKIAS
Length:420
Mass (Da):45,060
Last modified:May 27, 2002 - v2
Checksum:i87922DB2D73E1658
GO

Sequence cautioni

Q8YJ17: The sequence AAL51451 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51451.1. Different initiation.
PIRiAH3285.
RefSeqiWP_004684229.1. NZ_GG703781.1.

Genome annotation databases

EnsemblBacteriaiAAL51451; AAL51451; BMEI0269.
GeneIDi29593018.
KEGGibme:BMEI0269.
bmel:DK63_1163.
PATRICifig|224914.52.peg.1230.

Similar proteinsi

Entry informationi

Entry nameiISPG_BRUME
AccessioniPrimary (citable) accession number: Q8YJ17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: June 7, 2017
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families