Q8YJ17 (ISPG_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
EC=1.17.7.1

Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Gene names

Name:	ispG
Ordered Locus Names:	BMEI0269

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate By similarity. HAMAP MF_00159
Catalytic activity	(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin. HAMAP MF_00159
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00159
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6. HAMAP MF_00159
Sequence similarities	Belongs to the IspG family.
Sequence caution	The sequence AAL51451.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase HAMAP MF_00159		PRO_0000190547

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ17 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 87922DB2D73E1658	FASTA	420	45,060
	10 20 30 40 50 60 MSSETVSYFS HPFPRRQSVG VSVGGVIVGG SAPVVVQSMT NTDTADVDST VAQVAALHRA 70 80 90 100 110 120 GSEIVRITVD RDESAAAVPK IRERLERLGH DVPLVGDFHY IGHKLLADHP ACAEALAKYR 130 140 150 160 170 180 INPGNVGFKD KKDKQFADIV EMAIRYDKPV RIGVNWGSLD QELLTTLMDR NQAEGAPLSA 190 200 210 220 230 240 QDVMREAIVQ SALISANLAE EIGLGRDKII LSAKVSQVQD LIAVYTMLAQ RSNHALHLGL 250 260 270 280 290 300 TEAGMGTKGI VASSAAMGIL LQQGIGDTIR ISLTPEPGGD RTREVQVAQE LLQTMGFRQF 310 320 330 340 350 360 VPIVAACPGC GRTTSTVFQE LAQTIQEDIR RNMPLWREKY PGVEALSVAV MGCIVNGPGE 370 380 390 400 410 420 SKHADIGISL PGTGETPSAP VFVDGKKVTT LRGPGIAEDF QKMVADYIEN RFGLGRKIAS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51451.1. Different initiation.
PIR	AH3285.
RefSeq	NP_539187.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ17.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195981.
GenomeReviews	Gene locus BMEI0269 in contig AE008917_GR.
KEGG	bme:BMEI0269.
PATRIC	17850544. VBIBruMel92729_0294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335271.
OMA	PRKLEIV.
PhylomeDB	Q8YJ17.
ProtClustDB	PRK00366.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0269-MONOMER.
Family and domain databases
HAMAP	MF_00159. IspG. [Tree]
InterPro	IPR016425. IspG_bac. IPR004588. IspG_bac-typ. [Graphical view]
KO	K03526.
Pfam	PF04551. GcpE. 1 hit. [Graphical view]
PIRSF	PIRSF004640. IspG. 1 hit.
TIGRFAMs	TIGR00612. IspG_gcpE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPG_BRUME

Accession

Primary (citable) accession number: Q8YJ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program