Reviewed, UniProtKB/Swiss-Prot Q8YJ04 (THIQ_BRUME)

Names and origin

Protein names

Recommended name:
    Thiamine import ATP-binding protein thiQ
    EC=3.6.3.-

Gene names

Name:	thiQ
Ordered Locus Names:	BMEI0283

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the ABC transporter complex thiBPQ involved in thiamine import. Responsible for energy coupling to the transport system By similarity.
Subunit structure	The complex is composed of two ATP-binding proteins (thiQ), two transmembrane proteins (thiP) and a solute-binding protein (thiB) Probable.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Thiamine importer (TC 3.A.1.19.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin transport Inferred from electronic annotation. Source: HAMAP
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Thiamine import ATP-binding protein thiQ HAMAP MF_01723		PRO_0000274435
Regions
	Domain	7 – 235	229	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ04-1 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 585610441123F356	FASTA	241	26,171
	10 20 30 40 50 60 MTASADIRLS DVRFSYGETA MHFDVTITGG EIAAIVGPSG SGKSTFLNLI AGFETPQSGI 70 80 90 100 110 120 ISINGVDVTH LPPADRPVSM VFQENNLFAH LTVEQNVDLG RSPNLRLNEE DRKAVASALA 130 140 150 160 170 180 RVGLQGKEKR KPEALSGGER QRVAIARVLV RERPVLLLDE AFASLGPALR HQMLDLVNKL 190 200 210 220 230 240 RRETGMTVLM VTHTPEDALH LDALLIFLDN GKIAAQGPAT EMLSRAGPEA LRHYIGEMRS F

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51464.1. Different initiation.
PIR	AE3287.
RefSeq	NP_539200.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195994.
GenomeReviews	Gene locus BMEI0283 in contig AE008917_GR.
KEGG	bme:BMEI0283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJ04.
OMA	QDARRIG
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0283-MON.
Family and domain databases
HAMAP	MF_01723. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005968. ABC_transptr_thiamine_ATP-bd. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01277. thiQ. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51288. THIQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIQ_BRUME

Accession

Primary (citable) accession number: Q8YJ04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	November 24, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)