Reviewed, UniProtKB/Swiss-Prot Q8YJ03 (THIP_BRUME)

Names and origin

Protein names

Recommended name:
    Thiamine transport system permease protein thiP

Gene names

Name:	thiP
Ordered Locus Names:	BMEI0284

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane By similarity.
Subunit structure	The complex is composed of two ATP-binding proteins (thiQ), two transmembrane proteins (thiP) and a solute-binding protein (thiB) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 2 ABC transmembrane type-1 domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Thiamine transport system permease protein thiP		PRO_0000282909
Regions
	Transmembrane	19 – 39	21	Potential
	Transmembrane	64 – 84	21	Potential
	Transmembrane	102 – 122	21	Potential
	Transmembrane	142 – 162	21	Potential
	Transmembrane	205 – 225	21	Potential
	Transmembrane	250 – 270	21	Potential
	Transmembrane	300 – 320	21	Potential
	Transmembrane	343 – 363	21	Potential
	Transmembrane	379 – 399	21	Potential
	Transmembrane	406 – 426	21	Potential
	Transmembrane	468 – 488	21	Potential
	Transmembrane	510 – 530	21	Potential
	Domain	62 – 266	205	ABC transmembrane type-1 1
	Domain	339 – 530	192	ABC transmembrane type-1 2

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ03-1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 5FADDDB7893B96AA	FASTA	543	58,303
	10 20 30 40 50 60 MTATPARRTS LASPATKPVA GGLALAFLAT LAGGALLALA LEAGGGGFDA AANFDTYLWR 70 80 90 100 110 120 VARFTIWQAV ASSLLSVLFA IPIARALYAE ARFPGRGLIL RLFAQPLALP ALVAVLGVTS 130 140 150 160 170 180 IYGRNGLIAH ISDMLGHPMQ PDIYGIAGIL IAHIFFNMPL AVRLLLAAYE SIPDDHWKLA 190 200 210 220 230 240 AQLGMGSRAR FRLIDWPVIR RSLPGMIGLV FMLCVTSFTT VLTLGGGPRA TTLEVAIYQS 250 260 270 280 290 300 LHFDFDPARA VALTFTQLAL TLLILLILRL TGRPSEEGFT QTATPRRYGS PRKTERLFNI 310 320 330 340 350 360 IVIALGFLYV ALPIAGVVVS GLTADLVRLL SERIVWHAIA TSLALGFSAA LLAVFLSLAL 370 380 390 400 410 420 VAAREATRNA RIANIFDTGA SLILVMPPIV IGAGWFILLR HFTDPFVMAP LMVVTVNAAM 430 440 450 460 470 480 AMPFAVRLLR PAWDTAASRH NKLCSQLGIK GFNRLRLIDW PSIRRPCGMA FAFAMALSLG 490 500 510 520 530 540 DLGTIALFGS DALVTLPYLL LQRMGSYRTF DAAGLALILG VLCLALMMIA DRAAASRKEA FLQ

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51465.1. Different initiation.
PIR	AF3287.
RefSeq	NP_539201.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195995.
GenomeReviews	Gene locus BMEI0284 in contig AE008917_GR.
KEGG	bme:BMEI0284.
NMPDR	fig|224914.1.peg.283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296626.
OMA	SYRTEDA.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0284-MON.
Family and domain databases
InterPro	IPR000515. BPD_transp. IPR005947. ThiP_ABC_transpt. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIP_BRUME

Accession

Primary (citable) accession number: Q8YJ03

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	December 15, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)