Q8YJ02 (THIB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine-binding periplasmic protein

Gene names

Name:	thiB
Ordered Locus Names:	BMEI0285

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex thiBPQ involved in thiamine import By similarity.
Subunit structure	The complex is composed of two ATP-binding proteins (thiQ), two transmembrane proteins (thiP) and a solute-binding protein (thiB) Probable.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the bacterial solute-binding protein 1 family.
Sequence caution	The sequence AAL51466.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Periplasm
Domain	Signal
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 334	311	Thiamine-binding periplasmic protein		PRO_0000282913

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ02 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: E0AF4211C40716CC	FASTA	334	36,737
	10 20 30 40 50 60 MRLLSLLTFS LFAVIGLAPA AQAKDKLTIY TYDSFVSEWG PGPKVKENFE KECDCEVNFV 70 80 90 100 110 120 ASADGVALLN RLKLEGSKTA ADIVLGLDTN LTTEARASGF FAPSGIDQTN VKVPGNFKDD 130 140 150 160 170 180 IFVPYDYGYF AVVYDSEKLP NPPKSLKELV EGDPAQKIVL QDPRTATPGL GMLLWMKSVY 190 200 210 220 230 240 GDEAGAAWQK LQKRVLTVTP GWSEAYGLFT KGEAPMVLSY TTSPAYHMVV EKTNRYKALA 250 260 270 280 290 300 YPEGNYLQIE LAAQTTTGAK NPLAKKFLAF MTGPGFQDVI PETNWMFPAG KTSKPLPAAF 310 320 330 DALPKPEKTL LIPPYEVAKN RRLWVNEWLA ATSR

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51466.1. Different initiation.
PIR	AG3287.
RefSeq	NP_539202.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ02.
SMR	Q8YJ02. Positions 24-333.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195996.
GenomeReviews	Gene locus BMEI0285 in contig AE008917_GR.
KEGG	bme:BMEI0285.
NMPDR	fig|224914.1.peg.284.
PATRIC	17850582. VBIBruMel92729_0313.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476839.
OMA	IPTTNWM.
PhylomeDB	Q8YJ02.
ProtClustDB	PRK11205.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0285-MONOMER.
Family and domain databases
InterPro	IPR006059. Solute-bd_1_bac. IPR005948. Thi_ABC_peri-bd. IPR005967. ThiB_ABC_peri-bd. [Graphical view]
KO	K02064.
Pfam	PF01547. SBP_bac_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01254. SfuA. 1 hit. TIGR01276. ThiB. 1 hit.
PROSITE	PS01037. SBP_BACTERIAL_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIB_BRUME

Accession

Primary (citable) accession number: Q8YJ02

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program