Reviewed, UniProtKB/Swiss-Prot Q8YIY0 (PGK_BRUME)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	BMEI0309

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Phosphoglycerate kinase HAMAP MF_00145		PRO_0000145916
Regions
	Nucleotide binding	353 – 356	4	ATP By similarity
	Region	21 – 23	3	Substrate binding By similarity
	Region	59 – 62	4	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	201	1	ATP By similarity
	Binding site	323	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YIY0-1 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: D955AE611ACDCF9B	FASTA	396	41,810
	10 20 30 40 50 60 MMFRTLDDAN VQSKRVLVRV DLNVPMANGE VTDLTRIERI VPTIAELSRK GAKVILLAHF 70 80 90 100 110 120 GRPKGVASDE NSLKHVVKPL SKVLDHSVHF AEDCIGDKAK AAVDALKDGD VLLLENTRFH 130 140 150 160 170 180 KGEEKNDPEF VQALAANGDL YVNDAFSAAH RAHASTEGLA HVLPAFAGRA MQAELEALEK 190 200 210 220 230 240 GLGNPARPVV AIVGGAKVST KLDLLSNLIE KVDALVIGGG MANTFLAAKG LDVGKSLCEH 250 260 270 280 290 300 ELASTAREIM AKAETTKCAI ILPVDAIVGW HFAADTPHQT YGVDSVPGDA MILDAGELST 310 320 330 340 350 360 DLIASAIDDA ATLVWNGPLG AFELRPFDTA TVKVARHVAK RTKEGKLVSV GGGGDTVAAL 370 380 390 NHAGVADDFT YISTAGGAFL EWMEGKPLPG VDVLKK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51490.1. Different initiation.
PIR	AG3290.
RefSeq	NP_539226.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1196020.
GenomeReviews	Gene locus BMEI0309 in contig AE008917_GR.
KEGG	bme:BMEI0309.
NMPDR	fig|224914.1.peg.308.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YIY0.
OMA	FAADCVG
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0309-MON.
BRENDA	2.7.2.3. 277959.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_BRUME

Accession

Primary (citable) accession number: Q8YIY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 25, 2002
Last modified:	November 24, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)