Reviewed, UniProtKB/Swiss-Prot Q8YIV7 (RUVC_BRUME)

Names and origin

Protein names

Recommended name:
    Crossover junction endodeoxyribonuclease ruvC
    EC=3.1.22.4
Alternative name(s):
    Holliday junction nuclease ruvC
    Holliday junction resolvase ruvC

Gene names

Name:	ruvC
Ordered Locus Names:	BMEI0332

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the ruvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP
Molecular function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Crossover junction endodeoxyribonuclease ruvC HAMAP MF_00034		PRO_0000183081
Sites
	Metal binding	11	1	Magnesium By similarity
	Metal binding	71	1	Magnesium By similarity
	Metal binding	143	1	Magnesium By similarity
	Metal binding	146	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YIV7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C1CF7EAF34FF2F42	FASTA	173	18,455
	10 20 30 40 50 60 MKETIRIIGI DPGLRRTGWG IVESLGNSLH FIGSGTVTSN AEMDLASRLC QLHEGLSKVL 70 80 90 100 110 120 HEFMPHEAAV EHTFVNKDAT ATLKLGQARG IALLAPAQAG LPVAEYAPNA VKKAVIGVGH 130 140 150 160 170 GEKQQIHMMV KVLMPRASFD TSDAADALAI AICHAHHRQS IVSARRMQAL LAG

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51513.1.
PIR	AF3293.
RefSeq	NP_539249.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1196043.
GenomeReviews	Gene locus BMEI0332 in contig AE008917_GR.
KEGG	bme:BMEI0332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG437552.
OMA	RTGWGVI.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0332-MON.
BRENDA	3.1.22.4. 277959.
Family and domain databases
HAMAP	MF_00034. [Tree]
InterPro	IPR012337. PolynucTfrase_RNaseH_fold. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_BRUME

Accession

Primary (citable) accession number: Q8YIV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	March 1, 2002
Last modified:	December 15, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)