Q8YIV5 (RUVB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	BMEI0334

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday junction migration by localized denaturation and reannealing By similarity. HAMAP MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP MF_00016
Subunit structure	Forms a complex with RuvA By similarity. HAMAP MF_00016
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Holliday junction ATP-dependent DNA helicase RuvB HAMAP MF_00016		PRO_0000165505
Regions
	Nucleotide binding	59 – 66	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YIV5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F97710688F919FBC	FASTA	346	38,269
	10 20 30 40 50 60 MSDRNPLIDA DRRADEDNTL RPQTLDDFVG QAAARANLKV FIEAAKVRGE ALDHVLFVGP 70 80 90 100 110 120 PGLGKTTLAQ IMAKELGVNF RSTSGPVIAK AGDLAALLTN LEERDVLFID EIHRLSPAVE 130 140 150 160 170 180 EILYPAMEDF QLDLIIGEGP AARSVKIDLA KFTLVAATTR LGLLTTPLRD RFGIPVRLNF 190 200 210 220 230 240 YTVEELEYIV RRGARIMQMG ISSDGAREVA RRSRGTPRIV GRLLRRVRDF ALVAGADIID 250 260 270 280 290 300 RRIADEALSR LEVDNRGLDQ LDRRYLNIIA RNFGGGPVGI ETIAAGLSEP RDAIEDIIEP 310 320 330 340 YLIQQGFLQR TPRGRVLTAV AWQHLGLPAP AEIIQQSQYG LFMEDE

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51515.1.
PIR	AH3293.
RefSeq	NP_539251.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YIV5.
SMR	Q8YIV5. Positions 19-330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196045.
GenomeReviews	Gene locus BMEI0334 in contig AE008917_GR.
KEGG	bme:BMEI0334.
NMPDR	fig|224914.1.peg.333.
PATRIC	17802319. VBIBruMel146950_3476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG339960.
OMA	EKFDGGP.
PhylomeDB	Q8YIV5.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0334-MONOMER.
Family and domain databases
HAMAP	MF_00016. DNA_helic_RuvB. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR000641. CbxX_CfqX. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K03551.
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
PRINTS	PR00819. CBXCFQXSUPER.
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00635. RuvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_BRUME

Accession

Primary (citable) accession number: Q8YIV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program