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Protein

Malate synthase G

Gene

glcB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB), Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei281 – 2811Acetyl-CoAUniRule annotation
Binding sitei318 – 3181Acetyl-CoAUniRule annotation
Active sitei345 – 3451Proton acceptorUniRule annotation
Binding sitei345 – 3451GlyoxylateUniRule annotation
Metal bindingi437 – 4371MagnesiumUniRule annotation
Binding sitei437 – 4371GlyoxylateUniRule annotation
Metal bindingi465 – 4651MagnesiumUniRule annotation
Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei636 – 6361Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:BMEI0380
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 728728Malate synthase GPRO_0000166882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_3056.

Structurei

3D structure databases

ProteinModelPortaliQ8YIR3.
SMRiQ8YIR3. Positions 10-725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1312Acetyl-CoA bindingUniRule annotation
Regioni462 – 4654Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YIR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSAEKRNYV EIEGLAVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD
60 70 80 90 100
LAPKNRALLA KRDELQARID AWYKENRDKG YSQADYQQFL KDIGYLLPEG
110 120 130 140 150
GAFSVSTTNV DPEITHIAGP QLVVPVMNAR YALNAANARW GSLYDALYGT
160 170 180 190 200
DAISEADGAE KGKGYNPKRG EKVIAWAKNF LDESAPLSTG KWADVAGLAV
210 220 230 240 250
NDGKLEIRLT DGSATTLKDE SQFKGYNGDA ASPTNVLLAK HNMHVDIVIN
260 270 280 290 300
ADHPIGKTDP AHIADVVLES AISTIQDCED SIAAVDAEDK VAVYRNWLGL
310 320 330 340 350
MNGKLEDTFE KNGKQMTRRL NGDRTYTAPD GSTLTLKGRS LMLVRNVGHL
360 370 380 390 400
MTNPAILDAE GNEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK
410 420 430 440 450
PKMHGPEEVA FANEIFTRTE EMLGMKPNTL KIGIMDEERR TTVNLKEAIR
460 470 480 490 500
AAKDRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGDMKQAA WIGAYEQWNV
510 520 530 540 550
DIGLECGLSG HAQIGKGMWA MPDMMAAMLE QKIAHPKAGA NTAWVPSPTA
560 570 580 590 600
ATLHATHYHK IDVAAVQEKL KSRPRAKLDD ILSVPVAVRP NWTPDDIQHE
610 620 630 640 650
IDNNAQGILG YVVRWIDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW
660 670 680 690 700
LYHGVVSEAQ VMETMKRMAA IVDKQNEGDP LYRPMAADFD KSIAFQAACD
710 720
LVFKGREQPN GYTEPVLHRR RLELKQAS
Length:728
Mass (Da):79,986
Last modified:March 1, 2002 - v1
Checksum:iE1313A617979270D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51561.1.
PIRiAF3299.
RefSeqiWP_004684123.1. NZ_GG703781.1.

Genome annotation databases

EnsemblBacteriaiAAL51561; AAL51561; BMEI0380.
KEGGibme:BMEI0380.
PATRICi17802432. VBIBruMel146950_3531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51561.1.
PIRiAF3299.
RefSeqiWP_004684123.1. NZ_GG703781.1.

3D structure databases

ProteinModelPortaliQ8YIR3.
SMRiQ8YIR3. Positions 10-725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_3056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51561; AAL51561; BMEI0380.
KEGGibme:BMEI0380.
PATRICi17802432. VBIBruMel146950_3531.

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiMASZ_BRUME
AccessioniPrimary (citable) accession number: Q8YIR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.