Q8YIG1 (KPRS_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	BMEI0483

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAL51664.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141116
Regions
	Region	209 – 222	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	125	1	Magnesium Potential
	Metal binding	127	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YIG1 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: 6CED20B3AF31DD5E	FASTA	310	33,331
	10 20 30 40 50 60 MKLFAGNSNR VLAESVAQYL NIPLGKASVR RFADQEIFVE IQENVRGEDV FVLQSTSYPA 70 80 90 100 110 120 NDHLMELLIM IDAFRRSSAR RITAVLPYFG YARQDRKPGP RTPISAKLVA NLITEAGASR 130 140 150 160 170 180 VLTLDLHAGQ IQGFFDIPTD NLYAVPVIAR DVKANYATGN CMVVSPDVGG VVRARSLAKR 190 200 210 220 230 240 IDAQLAIVDK RRERPGESEV MNVIGDVSGK DCLLFDDIVD SGGTLCNAAE ALLNKGANSV 250 260 270 280 290 300 TAYITHGVLS GGAVARIASS KLKELVITDS IQPTTAINDA PNIRVLSISD LIGEAIARTA 310 AEESVSSLFD

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51664.1. Different initiation.
PIR	AE3312.
RefSeq	NP_539400.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YIG1.
SMR	Q8YIG1. Positions 1-308.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196194.
GenomeReviews	Gene locus BMEI0483 in contig AE008917_GR.
KEGG	bme:BMEI0483.
PATRIC	17851064. VBIBruMel92729_0542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q8YIG1.
ProtClustDB	PRK01259.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0483-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BRUME

Accession

Primary (citable) accession number: Q8YIG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program