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Q8YIB8

- CARA_BRUME

UniProt

Q8YIB8 - CARA_BRUME

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Protein
Carbamoyl-phosphate synthase small chain
Gene
carA, BMEI0526
Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei286 – 2861Nucleophile By similarity
Active sitei370 – 3701 By similarity
Active sitei372 – 3721 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
  3. carbamoyl phosphate biosynthetic process Source: InterPro
  4. glutamine catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Ordered Locus Names:BMEI0526
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Carbamoyl-phosphate synthase small chainUniRule annotation
PRO_0000112259Add
BLAST

Proteomic databases

PRIDEiQ8YIB8.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Protein-protein interaction databases

STRINGi224914.BMEI0526.

Structurei

3D structure databases

ProteinModelPortaliQ8YIB8.
SMRiQ8YIB8. Positions 16-396.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 397189Glutamine amidotransferase type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 205205CPSaseUniRule annotation
Add
BLAST

Sequence similaritiesi

Belongs to the CarA family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000038087.
KOiK01956.
OMAiIREKGMP.
OrthoDBiEOG61ZTH6.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YIB8-1 [UniParc]FASTAAdd to Basket

« Hide

MTETTPKTAP WTVQKRTAVL VLADGTVIEG KGLGATGAVE AEVVFNTALT    50
GYEEILTDPS YAGQIVTFTF PHIGNVGANA EDIEDLTPAN RHGAVGAIFK 100
ADITAPSNFR AAKDLDSWLK HRGIIALAGI DTRALTALIR ERGAQNAVIA 150
HDPNGNFDLD ALKARAANWC GLENLDLAKD VTIGQSLVWK ELPWTLQDGY 200
GEQDAPQYHV VALDFGVKRN ILRLLTGLGA KVTVLPATAT AEDVLAHNPD 250
GVFLSNGPGD PAATGEYAVP TIGKLVETGI PLFGICLGHQ MLALALGGRT 300
EKMHQGHHGA NHPVKDYTTG KVEIVSMNHG FAVDSDSLPE NVEETHVSLF 350
DGTNCGLRVV GKPVFSVQHH PEASPGPQDS HYLFRRFINL IRERKGQAPL 400
PEREQAA 407
Length:407
Mass (Da):43,536
Last modified:July 11, 2002 - v2
Checksum:iD6C01AFF0592F7E2
GO

Sequence cautioni

The sequence AAL51707.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51707.1. Different initiation.
PIRiAH3317.
RefSeqiNP_539443.2. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51707; AAL51707; BMEI0526.
GeneIDi1196237.
KEGGibme:BMEI0526.
PATRICi17851164. VBIBruMel92729_0592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51707.1 . Different initiation.
PIRi AH3317.
RefSeqi NP_539443.2. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YIB8.
SMRi Q8YIB8. Positions 16-396.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0526.

Proteomic databases

PRIDEi Q8YIB8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL51707 ; AAL51707 ; BMEI0526 .
GeneIDi 1196237.
KEGGi bme:BMEI0526.
PATRICi 17851164. VBIBruMel92729_0592.

Phylogenomic databases

HOGENOMi HOG000038087.
KOi K01956.
OMAi IREKGMP.
OrthoDBi EOG61ZTH6.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .

Miscellaneous databases

PROi Q8YIB8.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPi MF_01209. CPSase_S_chain.
InterProi IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view ]
Pfami PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view ]
SMARTi SM01097. CPSase_sm_chain. 1 hit.
[Graphical view ]
SUPFAMi SSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR01368. CPSaseIIsmall. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiCARA_BRUME
AccessioniPrimary (citable) accession number: Q8YIB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: June 11, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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