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Q8YIB8

- CARA_BRUME

UniProt

Q8YIB8 - CARA_BRUME

Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (11 Jul 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei286 – 2861NucleophileUniRule annotation
    Active sitei370 – 3701UniRule annotation
    Active sitei372 – 3721UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    2. arginine biosynthetic process Source: UniProtKB-HAMAP
    3. carbamoyl phosphate biosynthetic process Source: InterPro
    4. glutamine catabolic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00171.
    UPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
    Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
    Gene namesi
    Name:carAUniRule annotation
    Ordered Locus Names:BMEI0526
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Carbamoyl-phosphate synthase small chainPRO_0000112259Add
    BLAST

    Proteomic databases

    PRIDEiQ8YIB8.

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI0526.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YIB8.
    SMRiQ8YIB8. Positions 16-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini209 – 397189Glutamine amidotransferase type-1UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 205205CPSaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarA family.UniRule annotation
    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    HOGENOMiHOG000038087.
    KOiK01956.
    OMAiIREKGMP.
    OrthoDBiEOG61ZTH6.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPiMF_01209. CPSase_S_chain.
    InterProiIPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    [Graphical view]
    PfamiPF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view]
    SMARTiSM01097. CPSase_sm_chain. 1 hit.
    [Graphical view]
    SUPFAMiSSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YIB8-1 [UniParc]FASTAAdd to Basket

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    MTETTPKTAP WTVQKRTAVL VLADGTVIEG KGLGATGAVE AEVVFNTALT    50
    GYEEILTDPS YAGQIVTFTF PHIGNVGANA EDIEDLTPAN RHGAVGAIFK 100
    ADITAPSNFR AAKDLDSWLK HRGIIALAGI DTRALTALIR ERGAQNAVIA 150
    HDPNGNFDLD ALKARAANWC GLENLDLAKD VTIGQSLVWK ELPWTLQDGY 200
    GEQDAPQYHV VALDFGVKRN ILRLLTGLGA KVTVLPATAT AEDVLAHNPD 250
    GVFLSNGPGD PAATGEYAVP TIGKLVETGI PLFGICLGHQ MLALALGGRT 300
    EKMHQGHHGA NHPVKDYTTG KVEIVSMNHG FAVDSDSLPE NVEETHVSLF 350
    DGTNCGLRVV GKPVFSVQHH PEASPGPQDS HYLFRRFINL IRERKGQAPL 400
    PEREQAA 407
    Length:407
    Mass (Da):43,536
    Last modified:July 11, 2002 - v2
    Checksum:iD6C01AFF0592F7E2
    GO

    Sequence cautioni

    The sequence AAL51707.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51707.1. Different initiation.
    PIRiAH3317.
    RefSeqiNP_539443.2. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL51707; AAL51707; BMEI0526.
    GeneIDi1196237.
    KEGGibme:BMEI0526.
    PATRICi17851164. VBIBruMel92729_0592.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51707.1 . Different initiation.
    PIRi AH3317.
    RefSeqi NP_539443.2. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YIB8.
    SMRi Q8YIB8. Positions 16-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0526.

    Proteomic databases

    PRIDEi Q8YIB8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL51707 ; AAL51707 ; BMEI0526 .
    GeneIDi 1196237.
    KEGGi bme:BMEI0526.
    PATRICi 17851164. VBIBruMel92729_0592.

    Phylogenomic databases

    HOGENOMi HOG000038087.
    KOi K01956.
    OMAi IREKGMP.
    OrthoDBi EOG61ZTH6.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00171 .
    UPA00070 ; UER00115 .

    Miscellaneous databases

    PROi Q8YIB8.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPi MF_01209. CPSase_S_chain.
    InterProi IPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    [Graphical view ]
    Pfami PF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view ]
    SMARTi SM01097. CPSase_sm_chain. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiCARA_BRUME
    AccessioniPrimary (citable) accession number: Q8YIB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3