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Reviewed, UniProtKB/Swiss-Prot Q8YI68 (MURD_BRUME)

Last modified February 9, 2010. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme
Gene names
Name: murD
Ordered Locus Names: BMEI0577
OrganismBrucella melitensis [Complete proteome] [HAMAP]
Taxonomic identifier29459 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the murCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108982

Regions

Nucleotide binding121 – 1277ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q8YI68-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 4169640D6131198A

FASTA46748,782
        10         20         30         40         50         60 
MIPITALKDK TVALFGLGGS GIATAKAIVA GGARIIAWDD NPDSVARAQS AGIATGDLRQ 

        70         80         90        100        110        120 
ADWSQFAVFV LSPGVPLTHP QPHWSVDLAR AAGVEIIGDV ELFVRERNHI APDCPFIAIT 

       130        140        150        160        170        180 
GTNGKSTTTA LIAHIIKATG RDMQLGGNIG TAILTLELPC ADRFYVVECS SYQIDLAPSL 

       190        200        210        220        230        240 
NPTAGILLNL TPDHLDRHGS MENYAAIKER LVAASGTAII GIDDAYCQAI ADRLHGAGIR 

       250        260        270        280        290        300 
VVRISKEKHL DRGYFADGAK LLWAQDGEID EIASLEGIGS LRGAHNAQNA LAAIVACLSA 

       310        320        330        340        350        360 
GLSLEEIHAG LKSFPGLAHR MEQVGRRGKV LFVNDSKATN AEATAPALSS FPQNIYWIVG 

       370        380        390        400        410        420 
GVPKAGGINS LTAFFPRVAK AYLIGEAAAQ FAATLGGAVP FEISDTLAAA VAHAAGDAGN 

       430        440        450        460 
DAAPEPVVLL SPACASFDQF QNFEKRGDAF RDAVLALPGV MPMRGES 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51758.1.
PIRAC3324.
RefSeqNP_539494.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1196288.
GenomeReviewsGene locus BMEI0577 in contig AE008917_GR.
KEGGbme:BMEI0577.
NMPDRfig|224914.1.peg.576.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG750024.
OMAVKYYNDS.
PhylomeDBQ8YI68.

Enzyme and pathway databases

BioCycBMEL224914:BMEI0577-MONOMER.
BRENDA6.3.2.9. 277959.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01087. murD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BRUME
AccessionPrimary (citable) accession number: Q8YI68
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2002
Last modified: February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents