Q8YI64 (MURB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BMEI0581

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.
Sequence caution	The sequence AAL51762.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179185
Regions
	Domain	36 – 202	167	FAD-binding PCMH-type
Sites
	Active site	182	1	By similarity
	Active site	231	1	Proton donor By similarity
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YI64 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 35079AE908CE76D5	FASTA	322	34,891
	10 20 30 40 50 60 MMESGEALLK KLDGRLSGLR GRLTPDTGMD KITWFRAGGP AQVLFQPSDE EDLSAFLKAV 70 80 90 100 110 120 PEEIPLLVVG IGSNLLVRDG GVPGFVVRLS AKGFGEVEQV CDTQLRAGAA APDKRVAAAA 130 140 150 160 170 180 LEAGLAGFHF YHGIPGGIGG ALRMNAGANG VETRERVVEV RALDRKGEVH VLSNADMGYA 190 200 210 220 230 240 YRHSSASPDL IFTSVLFEGV PGERDDIRRA MDEVQHHRET VQPVREKTGG STFKNPEGTS 250 260 270 280 290 300 AWKEIDKAGC RGLRVGGAQM SEMHCNFMIN TGNATGHDLE TLGETVRARV FENSGIRLHW 310 320 EIKRLGLFRE GEQIEEFLGK IV

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51762.1. Different initiation.
PIR	AG3324.
RefSeq	NP_539498.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YI64.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196292.
GenomeReviews	Gene locus BMEI0581 in contig AE008917_GR.
KEGG	bme:BMEI0581.
NMPDR	fig|224914.1.peg.580.
PATRIC	17851285. VBIBruMel92729_0651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	SKKHAGF.
PhylomeDB	Q8YI64.
ProtClustDB	PRK13905.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0581-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BRUME

Accession

Primary (citable) accession number: Q8YI64

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	April 30, 2003
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program