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Q8YI63 (DDLB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase B

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase B
D-alanylalanine synthetase B
Gene names
Name:ddlB
Ordered Locus Names:BMEI0582
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315D-alanine--D-alanine ligase B HAMAP-Rule MF_00047
PRO_0000177795

Regions

Domain109 – 309201ATP-grasp
Nucleotide binding135 – 19056ATP By similarity

Sites

Metal binding2591Magnesium or manganese 1 By similarity
Metal binding2761Magnesium or manganese 1 By similarity
Metal binding2761Magnesium or manganese 2 By similarity
Metal binding2781Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YI63 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: AA15B74236EE24F8

FASTA31534,269
        10         20         30         40         50         60 
MRRGEARMTG KHVAVLMGGF SSERSVSLSS GVACATTLEE CGYRVTRIDV DRNVASILVE 

        70         80         90        100        110        120 
LKPDVVFNAL HGPFGEDGAI QGVLEYLQIP YTHSGVLASA LAMDKDRAKK VAAAAGVVVA 

       130        140        150        160        170        180 
PSRLMNRFDI GSQHPMKPPY VVKPVREGSS FGVVIVKEDQ PHPPQVIGSA DWKYGDEVMV 

       190        200        210        220        230        240 
EGYIAGRELT CAVMGDRAMD VCEIMPVGYQ FYDYDSKYVA GASTHVCPAK ILPNIYQKIQ 

       250        260        270        280        290        300 
TMALTAHRAI GCRGVSRSDF RFDDRFSEEG EVVWLEINTQ PGMTPTSLVP DIAKAAGISF 

       310 
AELLSWMVED ASCLR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51763.1.
PIRAH3324.
RefSeqNP_539499.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YI63.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0582.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51763; AAL51763; BMEI0582.
GeneID1196293.
KEGGbme:BMEI0582.
PATRIC17851287. VBIBruMel92729_0652.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-604-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLB_BRUME
AccessionPrimary (citable) accession number: Q8YI63
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names