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Reviewed, UniProtKB/Swiss-Prot Q8YI63 (DDLB_BRUME)

Last modified November 25, 2008. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    D-alanine--D-alanine ligase B
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase B
    D-Ala-D-Ala ligase B
Gene names
Name: ddlB
Ordered Locus Names: BMEI0582
OrganismBrucella melitensis [Complete proteome] [HAMAP]
Taxonomic identifier29459 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Cell wall formation By similarity.

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

CytoplasmBy similarity.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315D-alanine--D-alanine ligase B
PRO_0000177795

Regions

Domain109 – 309201ATP-grasp
Nucleotide binding135 – 19056ATP By similarity

Sites

Metal binding2591Magnesium or manganese 1 By similarity
Metal binding2761Magnesium or manganese 1 By similarity
Metal binding2761Magnesium or manganese 2 By similarity
Metal binding2781Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YI63-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: AA15B74236EE24F8

FASTA31534,269
        10         20         30         40         50         60 
MRRGEARMTG KHVAVLMGGF SSERSVSLSS GVACATTLEE CGYRVTRIDV DRNVASILVE 

        70         80         90        100        110        120 
LKPDVVFNAL HGPFGEDGAI QGVLEYLQIP YTHSGVLASA LAMDKDRAKK VAAAAGVVVA 

       130        140        150        160        170        180 
PSRLMNRFDI GSQHPMKPPY VVKPVREGSS FGVVIVKEDQ PHPPQVIGSA DWKYGDEVMV 

       190        200        210        220        230        240 
EGYIAGRELT CAVMGDRAMD VCEIMPVGYQ FYDYDSKYVA GASTHVCPAK ILPNIYQKIQ 

       250        260        270        280        290        300 
TMALTAHRAI GCRGVSRSDF RFDDRFSEEG EVVWLEINTQ PGMTPTSLVP DIAKAAGISF 

       310 
AELLSWMVED ASCLR 

« Hide

Cross-references

Sequence databases

AE009500 Genomic DNA. Translation: AAL51763.1.
PIRAH3324.
RefSeqNP_539499.1.

3D structure databases

HSSPHSSP built from PDB template 1IOW based on UniProtKB P07862.
ModBaseSearch...

Genome annotation databases

GeneID1196293.
GenomeReviewsGene locus BMEI0582 in contig AE008917_GR.
KEGGbme:BMEI0582.
NMPDRfig|224914.1.peg.581.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8YI63.

Enzyme and pathway databases

BioCycBMEL224914:BMEI0582-MON.

Family and domain databases

HAMAPMF_00047.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR013817. Pre-ATP_grasp.
[Graphical view]
Gene3DG3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLB_BRUME
AccessionPrimary (citable) accession number: Q8YI63
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2002
Last modified: November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents