Q8YI21 (ILVC_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacyl reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BMEI0624

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP-Rule MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP-Rule MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ketol-acid reductoisomerase HAMAP-Rule MF_00435		PRO_0000151284
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YI21 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1A4967D9205EEE92	FASTA	339	37,080
	10 20 30 40 50 60 MRVYYDRDAD VNLIKSKKVV IVGYGSQGRA HALNLKDSGA ANVRVALREG SATVQKAQAD 70 80 90 100 110 120 GFEVMNVADA AKWADLMMMA TPDELQADIY RDHIHNNLRD GAAIAFAHGL NVHCGLIEPK 130 140 150 160 170 180 KTVDVMMIAP KGPGHTVRGE YQKGGGVPCL IAIHQDASGN AHDLALSYAS GVGGGRSGVI 190 200 210 220 230 240 ETTFKEECET DLFGEQAVLC GGVVELIRTG FEVLVEAGYA PEMAYFECLN EMKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEWGEY VTGPRIITAE TKAEMKRVLK DIQTGKFTSD WMQEWKAGAA 310 320 330 RFKGIRRLND AHQIEEVGGK LRAMMPWIEK NKLVDKARN

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51805.1.
PIR	AB3330.
RefSeq	NP_539541.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YI21.
SMR	Q8YI21. Positions 1-328.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0624.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51805; AAL51805; BMEI0624.
GeneID	1196335.
KEGG	bme:BMEI0624.
PATRIC	17801362. VBIBruMel146950_3006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000016230.
KO	K00053.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
UniPathway	UPA00047; UER00056. UPA00049; UER00060.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit.
HAMAP	MF_00435. IlvC.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
PANTHER	PTHR21371. PTHR21371. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BRUME

Accession

Primary (citable) accession number: Q8YI21

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program