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Protein

Urease subunit alpha 2

Gene

ureC2

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta 1 (ureB1), Urease subunit alpha 2 (ureC2), Urease subunit beta 2 (ureB2), Urease subunit gamma 2 (ureA2), Urease subunit alpha 1 (ureC1), Urease subunit gamma 1 (ureA1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi140Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi142Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi223Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi223Nickel 2; via carbamate groupUniRule annotation1
Binding sitei225SubstrateUniRule annotation1
Metal bindingi252Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi278Nickel 2; via tele nitrogenUniRule annotation1
Active sitei326Proton donorUniRule annotation1
Metal bindingi366Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciBMEL224914:G1FZL-693-MONOMER
UniPathwayiUPA00258; UER00370

Protein family/group databases

MEROPSiM38.982

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alpha 2UniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alpha 2UniRule annotation
Gene namesi
Name:ureC2UniRule annotation
Ordered Locus Names:BMEI0647
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002341441 – 573Urease subunit alpha 2Add BLAST573

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiQ8YHZ8

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_2622

Structurei

3D structure databases

ProteinModelPortaliQ8YHZ8
SMRiQ8YHZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 573UreaseUniRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM Bacteria
COG0804 LUCA
HOGENOMiHOG000075064
KOiK01428
OMAiCHHLSHD

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PRINTSiPR01752 UREASE
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 2 hits
TIGRFAMsiTIGR01792 urease_alph, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YHZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQISRQQYA DLYGPTIGDK IRLGDSDLYV EIEKDLRATY GDELQYGGGK
60 70 80 90 100
TLRDGMGSEN FLTQEAGCLD LVITNVTVID AIQGVVKADV GIRNGRIVGL
110 120 130 140 150
GKAGNPSTMD GVTRGLVTGA STDAISGEHL ILTAGGMDTH VHYIAPQQVE
160 170 180 190 200
AALSNGITTL WGGGIGPVDG TNGVTTTNGP WNLEMMLRSI EGLPINFGIQ
210 220 230 240 250
GKGNSTGIAP LIEQLEAGAA GFKVHEDYGA TPATIRACLS VADEYDVSVA
260 270 280 290 300
VHTDTLNESG YVEDTIAAFD GRSVHTYHSE GAGGGHAPDL LKVVGQNNIL
310 320 330 340 350
PSSTNPTLPC GKNSVAELFD MIMVCHNLNP RIPSDVAFAE SRVRAETIVA
360 370 380 390 400
ESVLHDMGAI SMIGSDSQAM GRIGETYLRA IQTADAMKKA RGPLPEDAPG
410 420 430 440 450
NDNFRVLRYI AKVTINPALT AGVGDVIGSI ESGKFADLVL WEPAFFGVKP
460 470 480 490 500
KLVLKGGLVA WANMGDPNAS LPTPQPMYYR PMFAAYGSAL QKTSITFVSR
510 520 530 540 550
AAYDKGVADR FGLQRLVMPV SGTRVIGKAH MVRNSYLPNI EVDPQTFAVK
560 570
VDGVHATVKP PQSISLNQLY FFS
Length:573
Mass (Da):60,785
Last modified:March 1, 2002 - v1
Checksum:iA297E706F15A490B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL51828.1
PIRiAI3332
RefSeqiWP_004683985.1, NZ_GG703780.1

Genome annotation databases

EnsemblBacteriaiAAL51828; AAL51828; BMEI0647
GeneIDi29593437
KEGGibme:BMEI0647
bmel:DK63_780
PATRICifig|224914.52.peg.817

Similar proteinsi

Entry informationi

Entry nameiURE12_BRUME
AccessioniPrimary (citable) accession number: Q8YHZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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