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Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi300Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 2UniRule annotation1
Metal bindingi315Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 224ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEL224914:G1FZL-775-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase AUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligase AUniRule annotation
D-alanylalanine synthetaseAUniRule annotation
Gene namesi
Name:ddlAUniRule annotation
Ordered Locus Names:BMEI0727
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001777941 – 353D-alanine--D-alanine ligase AAdd BLAST353

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_2701

Structurei

3D structure databases

ProteinModelPortaliQ8YHR9
SMRiQ8YHR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 346ATP-graspUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
KOiK01921
OMAiRCDFFVI

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YHR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ
60 70 80 90 100
WLLSRFVNGA LEKPSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP
110 120 130 140 150
VLHGLHGEDG AVQGLAQVAR VPLAGCGIPG SANALDKDIA KRLVNEAGLS
160 170 180 190 200
TAKSVTITRE EVPAFSALEQ ALGLPIFIKP ARQGSSVGVH KVVTEADYQA
210 220 230 240 250
AMSDGFIYDD KLLAEEFIQA REVECGVLED EGGALFVSRA GEIVPAESHC
260 270 280 290 300
FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIAAKAFRV LGCDSMARVD
310 320 330 340 350
FFVTADRRIV LNEINTIPGF TDMSMYFKVM AVSGVSYPEI INRLVAHGLA

RGS
Length:353
Mass (Da):37,574
Last modified:October 31, 2003 - v2
Checksum:i058DAEA09A676155
GO

Sequence cautioni

The sequence AAL51908 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL51908.1 Different initiation.
PIRiAI3342
RefSeqiWP_004683939.1, NZ_GG703780.1

Genome annotation databases

EnsemblBacteriaiAAL51908; AAL51908; BMEI0727
GeneIDi29593528
KEGGibme:BMEI0727
bmel:DK63_699
PATRICifig|224914.52.peg.729

Similar proteinsi

Entry informationi

Entry nameiDDLA_BRUME
AccessioniPrimary (citable) accession number: Q8YHR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health