Reviewed, UniProtKB/Swiss-Prot Q8YHR9 (DDLA_BRUME)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase A
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetaseA
    D-Ala-D-Ala ligase A

Gene names

Name:	ddlA
Ordered Locus Names:	BMEI0727

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	D-alanine--D-alanine ligase A HAMAP MF_00047		PRO_0000177794
Regions
	Domain	141 – 346	206	ATP-grasp
	Nucleotide binding	169 – 224	56	ATP By similarity
Sites
	Metal binding	300	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 2 By similarity
	Metal binding	315	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHR9-1 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: 058DAEA09A676155	FASTA	353	37,574
	10 20 30 40 50 60 MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ WLLSRFVNGA 70 80 90 100 110 120 LEKPSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP VLHGLHGEDG AVQGLAQVAR 130 140 150 160 170 180 VPLAGCGIPG SANALDKDIA KRLVNEAGLS TAKSVTITRE EVPAFSALEQ ALGLPIFIKP 190 200 210 220 230 240 ARQGSSVGVH KVVTEADYQA AMSDGFIYDD KLLAEEFIQA REVECGVLED EGGALFVSRA 250 260 270 280 290 300 GEIVPAESHC FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIAAKAFRV LGCDSMARVD 310 320 330 340 350 FFVTADRRIV LNEINTIPGF TDMSMYFKVM AVSGVSYPEI INRLVAHGLA RGS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009514 Genomic DNA. Translation: AAL51908.1. Different initiation.
PIR	AI3342.
RefSeq	NP_539644.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOV based on UniProtKB P07862.
ModBase	Search...
Genome annotation databases
GeneID	1196438.
GenomeReviews	Gene locus BMEI0727 in contig AE008917_GR.
KEGG	bme:BMEI0727.
NMPDR	fig|224914.1.peg.726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YHR9.
OMA	Q8YHR9. GREIECG.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0727-MON.
BRENDA	6.3.2.4. 277959.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLA_BRUME

Accession

Primary (citable) accession number: Q8YHR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)