Q8YHR9 (DDLA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase A
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetaseA

Gene names

Name:	ddlA
Ordered Locus Names:	BMEI0727

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAL51908.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	D-alanine--D-alanine ligase A HAMAP-Rule MF_00047		PRO_0000177794
Regions
	Domain	141 – 346	206	ATP-grasp
	Nucleotide binding	169 – 224	56	ATP By similarity
Sites
	Metal binding	300	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 1 By similarity
	Metal binding	313	1	Magnesium or manganese 2 By similarity
	Metal binding	315	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHR9 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: 058DAEA09A676155	FASTA	353	37,574
	10 20 30 40 50 60 MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ WLLSRFVNGA 70 80 90 100 110 120 LEKPSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP VLHGLHGEDG AVQGLAQVAR 130 140 150 160 170 180 VPLAGCGIPG SANALDKDIA KRLVNEAGLS TAKSVTITRE EVPAFSALEQ ALGLPIFIKP 190 200 210 220 230 240 ARQGSSVGVH KVVTEADYQA AMSDGFIYDD KLLAEEFIQA REVECGVLED EGGALFVSRA 250 260 270 280 290 300 GEIVPAESHC FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIAAKAFRV LGCDSMARVD 310 320 330 340 350 FFVTADRRIV LNEINTIPGF TDMSMYFKVM AVSGVSYPEI INRLVAHGLA RGS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51908.1. Different initiation.
PIR	AI3342.
RefSeq	NP_539644.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YHR9.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51908; AAL51908; BMEI0727.
GeneID	1196438.
KEGG	bme:BMEI0727.
PATRIC	17851623. VBIBruMel92729_0818.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000011593.
KO	K01921.
OMA	LLHGPFG.
ProtClustDB	PRK01966.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLA_BRUME

Accession

Primary (citable) accession number: Q8YHR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program