Q8YHH2 (CDSA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidate cytidylyltransferase
EC=2.7.7.41

Alternative name(s):
CDP-DAG synthase
CDP-DG synthase
CDP-diacylglycerol synthase
Short name=CDS
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
CTP:phosphatidate cytidylyltransferase

Gene names

Name:	cdsA
Ordered Locus Names:	BMEI0828

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	CTP + phosphatidate = diphosphate + CDP-diacylglycerol.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the CDS family.
Sequence caution	The sequence AAL52009.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Phosphatidate cytidylyltransferase		PRO_0000090729
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Transmembrane	248 – 268	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YHH2 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: 2A2923106D71A723	FASTA	270	28,432
	10 20 30 40 50 60 MSNLQTRIIT AIVLGTITLW LTWVGGVGFT LFSIAIGLAM FYEWTELSAT RQTAFSRLFG 70 80 90 100 110 120 WAWLIVTGIL LILDRGALLT IGFLVAGCAI LLVTQWKSGR GWPAAGLFYA GFSALSLSLL 130 140 150 160 170 180 RGDEPFGFTT IVFLFAVVWS TDIAAYFNGR ALGGPKLAPR FSPNKTWSGA IGGAAAAVTG 190 200 210 220 230 240 GLLVASLVAA PGGWGVPVLA LLLSIVSQIG DLAESWVKRQ FGAKDSGRLL PGHGGVLDRV 250 260 270 DGLVAAAALL YLFGAIFAEP DVPSAIFFSF

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52009.1. Different initiation.
PIR	AF3355.
RefSeq	NP_539745.1. NC_003317.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196539.
GenomeReviews	Gene locus BMEI0828 in contig AE008917_GR.
KEGG	bme:BMEI0828.
NMPDR	fig|224914.1.peg.827.
PATRIC	17851861. VBIBruMel92729_0934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732679.
OMA	DILAYFV.
ProtClustDB	CLSK898223.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0828-MONOMER.
Family and domain databases
InterPro	IPR000374. PC_trans. [Graphical view]
KO	K00981.
Pfam	PF01148. CTP_transf_1. 1 hit. [Graphical view]
PROSITE	PS01315. CDS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDSA_BRUME

Accession

Primary (citable) accession number: Q8YHH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program