ID   Q8YHG5_BRUME            Unreviewed;       437 AA.
AC   Q8YHG5;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 130.
DE   RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN   OrderedLocusNames=BMEI0836 {ECO:0000313|EMBL:AAL52017.1};
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914 {ECO:0000313|EMBL:AAL52017.1, ECO:0000313|Proteomes:UP000000419};
RN   [1] {ECO:0000313|EMBL:AAL52017.1, ECO:0000313|Proteomes:UP000000419}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094
RC   {ECO:0000313|Proteomes:UP000000419};
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.J.,
RA   Haselkorn R., Kyrpides N., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00000308,
CC         ECO:0000256|RuleBase:RU003370};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC       ECO:0000256|RuleBase:RU003370}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family.
CC       {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC       ECO:0000256|RuleBase:RU003406}.
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DR   EMBL; AE008917; AAL52017.1; -; Genomic_DNA.
DR   PIR; AF3356; AF3356.
DR   AlphaFoldDB; Q8YHG5; -.
DR   KEGG; bme:BMEI0836; -.
DR   eggNOG; COG0372; Bacteria.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 2.20.28.60; -; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   NCBIfam; TIGR01798; cit_synth_I; 1.
DR   PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000313|EMBL:AAL52017.1};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW   ECO:0000256|RuleBase:RU003370}.
FT   ACT_SITE        313
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT   ACT_SITE        371
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ   SEQUENCE   437 AA;  49034 MW;  FCCFF0970F123547 CRC64;
     MDMKGKTMSD KTASFTLDGK TFDLPVRKGT VGPDVVDIGP LYKNSHAFTY DPGFTSTASC
     ESKITYIDGD EGVLLYRGYP IDQLAEHGDF LETCYLLLYG ELPTATQKAD FDYRVTRHTM
     LHEQMTKFFT GFRRDAHPMA VMVGCLGAMS AFYHDSTDIT DPHQRMVASI RLIAKVPTLA
     AMAYKYHIGQ PFVYPKNDLD FASNFLHMCF TVPCEEYKVN PVLARAMDRI FILHADHEQN
     ASTSTVRLAG SSGANPFACI AAGVACLWGP AHGGANEAAL NMLSEIGSVD RIPEYIARAK
     DKNDPFRLMG FGHRVYKNYD PRAKIMQKTC HEVLGELGIK DDPLLDVAME LERIALTDEY
     FIEKKLYPNI DFYSGITLKA LGFPTEMFTV LFALARTVGW VAQWKEMIED PQQKIGRPRQ
     LYTGATERDY VPLAKRK
//