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Q8YHG4

- SYE1_BRUME

UniProt

Q8YHG4 - SYE1_BRUME

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Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-864-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 1UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 1UniRule annotation
Short name:
GluRS 1UniRule annotation
Gene namesi
Name:gltX1UniRule annotation
Ordered Locus Names:BMEI0837
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Glutamate--tRNA ligase 1PRO_0000119522Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BMEI0837.

Structurei

3D structure databases

ProteinModelPortaliQ8YHG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi240 – 2445"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252722.
KOiK01885.
OMAiAMGWEVP.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YHG4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKPVITRFA PSPTGYLHIG GARTALFNWL YAKHCGGKML LRIEDTDRER
60 70 80 90 100
STEAATAAIL DGLTWLGLDW DGEAISQFER APRHREVAEE LVANGKAYYC
110 120 130 140 150
YASPEELEEM REKARAEGRP PRYDGRWRDR DPSEAPAGVK PVIRIKAPRD
160 170 180 190 200
GETVVHDAVQ GDVRFPNKDL DDFIILRSDG TPTYMHAVVV DDHDMGVTHI
210 220 230 240 250
IRGDDHLTNA ARQTIIYNAM GWDVPQMSHI PLIHGADGAK LSKRHGALGV
260 270 280 290 300
DAYRAIGYLP AALRNYLVRL GWSHGDDEIM STEQMIEWFD VKDINKGAAR
310 320 330 340 350
FDFQKLEAIN GLYMRSSDDQ ALFDALVAVL PEIEGGKELA EALDDKGRAQ
360 370 380 390 400
LLLAMPGLKE RAKTLVELAD GAKFIFASRP LALDEKAASL LNDEGRAVLK
410 420 430 440 450
PVYPVLEAMG EWTAESLDAA IRAHAEAEGL KLGKIAQPLR AALTGRATSP
460 470
GVFDVLVVLG REESLARIGD QIG
Length:473
Mass (Da):52,063
Last modified:April 23, 2003 - v2
Checksum:iF7F4A7FB7E42E5D0
GO

Sequence cautioni

The sequence AAL52018.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52018.1. Different initiation.
PIRiAG3356.
RefSeqiNP_539754.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52018; AAL52018; BMEI0837.
GeneIDi1196548.
KEGGibme:BMEI0837.
PATRICi17851879. VBIBruMel92729_0943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52018.1 . Different initiation.
PIRi AG3356.
RefSeqi NP_539754.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YHG4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0837.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL52018 ; AAL52018 ; BMEI0837 .
GeneIDi 1196548.
KEGGi bme:BMEI0837.
PATRICi 17851879. VBIBruMel92729_0943.

Phylogenomic databases

HOGENOMi HOG000252722.
KOi K01885.
OMAi AMGWEVP.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci BMEL224914:GCJ0-864-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiSYE1_BRUME
AccessioniPrimary (citable) accession number: Q8YHG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3