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Q8YHG4

- SYE1_BRUME

UniProt

Q8YHG4 - SYE1_BRUME

Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei243 – 2431ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-864-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 1UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 1UniRule annotation
    Short name:
    GluRS 1UniRule annotation
    Gene namesi
    Name:gltX1UniRule annotation
    Ordered Locus Names:BMEI0837
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Glutamate--tRNA ligase 1PRO_0000119522Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI0837.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YHG4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi11 – 2111"HIGH" regionAdd
    BLAST
    Motifi240 – 2445"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiAMGWEVP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YHG4-1 [UniParc]FASTAAdd to Basket

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    MSKPVITRFA PSPTGYLHIG GARTALFNWL YAKHCGGKML LRIEDTDRER    50
    STEAATAAIL DGLTWLGLDW DGEAISQFER APRHREVAEE LVANGKAYYC 100
    YASPEELEEM REKARAEGRP PRYDGRWRDR DPSEAPAGVK PVIRIKAPRD 150
    GETVVHDAVQ GDVRFPNKDL DDFIILRSDG TPTYMHAVVV DDHDMGVTHI 200
    IRGDDHLTNA ARQTIIYNAM GWDVPQMSHI PLIHGADGAK LSKRHGALGV 250
    DAYRAIGYLP AALRNYLVRL GWSHGDDEIM STEQMIEWFD VKDINKGAAR 300
    FDFQKLEAIN GLYMRSSDDQ ALFDALVAVL PEIEGGKELA EALDDKGRAQ 350
    LLLAMPGLKE RAKTLVELAD GAKFIFASRP LALDEKAASL LNDEGRAVLK 400
    PVYPVLEAMG EWTAESLDAA IRAHAEAEGL KLGKIAQPLR AALTGRATSP 450
    GVFDVLVVLG REESLARIGD QIG 473
    Length:473
    Mass (Da):52,063
    Last modified:April 23, 2003 - v2
    Checksum:iF7F4A7FB7E42E5D0
    GO

    Sequence cautioni

    The sequence AAL52018.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52018.1. Different initiation.
    PIRiAG3356.
    RefSeqiNP_539754.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52018; AAL52018; BMEI0837.
    GeneIDi1196548.
    KEGGibme:BMEI0837.
    PATRICi17851879. VBIBruMel92729_0943.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52018.1 . Different initiation.
    PIRi AG3356.
    RefSeqi NP_539754.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YHG4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52018 ; AAL52018 ; BMEI0837 .
    GeneIDi 1196548.
    KEGGi bme:BMEI0837.
    PATRICi 17851879. VBIBruMel92729_0943.

    Phylogenomic databases

    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi AMGWEVP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci BMEL224914:GCJ0-864-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiSYE1_BRUME
    AccessioniPrimary (citable) accession number: Q8YHG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3