Q8YHF5 (TPIS1_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase 1
Short name=TIM 1
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase 1

Gene names

Name:	tpiA1
Ordered Locus Names:	BMEI0846

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Triosephosphate isomerase 1 HAMAP MF_00147_B		PRO_0000090191
Sites
	Active site	99	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	12	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHF5 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: A0A54A4125082027	FASTA	254	26,499
	10 20 30 40 50 60 MTPGIRPLVA GNWKMNGKGE SLTELRAIAA GLSSDLGRKL DAVICVPATL LSRAAETLEG 70 80 90 100 110 120 ETVGLGGQDA HFKTSGAHTG DISPEMLKEA GATHVILGHS ERRTDHHESN KLICAKTEAA 130 140 150 160 170 180 WAAGLVAIVC VGETASERKA ERALDVIGDQ LSGSLPDGVT AENTIIAYEP IWAIGTGLTP 190 200 210 220 230 240 TVQDVRAAHA FMREQLIERF GAKGAHLRLL YGGSVKPSNA AELLGVADVD GALVGGASLK 250 AADFLAICET YRNL

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52027.1.
PIR	AH3357.
RefSeq	NP_539763.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YHF5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196557.
GenomeReviews	Gene locus BMEI0846 in contig AE008917_GR.
KEGG	bme:BMEI0846.
NMPDR	fig|224914.1.peg.845.
PATRIC	17801869. VBIBruMel146950_3254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708281.
OMA	ANTVIAY.
PhylomeDB	Q8YHF5.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0846-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS1_BRUME

Accession

Primary (citable) accession number: Q8YHF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program