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Q8YHF2

- PYRG_BRUME

UniProt

Q8YHF2 - PYRG_BRUME

Protein

CTP synthase

Gene

pyrG

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

    Catalytic activityi

    ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

    Enzyme regulationi

    Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei380 – 3801NucleophileUniRule annotation
    Active sitei514 – 5141UniRule annotation
    Active sitei516 – 5161UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. CTP synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' CTP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00159; UER00277.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
    Alternative name(s):
    CTP synthetaseUniRule annotation
    UTP--ammonia ligaseUniRule annotation
    Gene namesi
    Name:pyrGUniRule annotation
    Ordered Locus Names:BMEI0849
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542CTP synthasePRO_0000138167Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI0849.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YHF2.
    SMRiQ8YHF2. Positions 2-540.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini291 – 541251Glutamine amidotransferase type-1UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 252252Aminator domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CTP synthase family.UniRule annotation
    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    HOGENOMiHOG000077515.
    KOiK01937.
    OMAiYWNVISK.
    OrthoDBiEOG6RC3NR.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPiMF_01227. PyrG.
    InterProiIPR029062. Class_I_gatase-like.
    IPR004468. CTP_synthase.
    IPR017456. CTP_synthase_N.
    IPR017926. GATASE.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11550. PTHR11550. 1 hit.
    PfamiPF06418. CTP_synth_N. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00337. PyrG. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YHF2-1 [UniParc]FASTAAdd to Basket

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    MARYVFITGG VVSSLGKGIA AAALAALLQA RGYRVRIRKL DPYLNVDPGT    50
    MSPYQHGEVF VTDDGAETDL DLGHYERFTG RPANQQDNIT TGRIYRNIIE 100
    KERRGDYLGA TVQVIPHVTD EIKNFVLEGN EDYDFVLCEI GGTVGDIEAM 150
    PFLEAIRQLG NELPRGTAVY IHLTLMPYIP AAGELKTKPT QHSVKELRSI 200
    GIAPDILLVR ADREIPESER RKLSLFCNVR ESAVIQALDV ATIYDVPIAY 250
    HKEGLDSEVL SAFGIDPAPK PRMDRWEEVS HRLHNPEGEV TIAVVGKYTG 300
    LKDAYKSLIE ALHHGGLANK VKVNLDWIEA EVFESEDPAP YLEKVHGILV 350
    PGGFGERGAE GKILAAKFAR ERKVPYFGIC FGMQMACIEA ARNLVGIEDA 400
    SSSEFDPTRE PVVGLMTEWL KGNMLEKRAA AGDLGGTMRL GAYEAVLKPD 450
    SKIAQIYGST DIHERHRHRY EVNIDYKDRL EAAGLNFAGM SPDGVLPETV 500
    EYADHPWFIG VQYHPELKSR PFEPHPLFAS FIEAAIEQSR LV 542
    Length:542
    Mass (Da):60,135
    Last modified:March 1, 2002 - v1
    Checksum:i30FE1EB1F908493E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52030.1.
    PIRiAC3358.
    RefSeqiNP_539766.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52030; AAL52030; BMEI0849.
    GeneIDi1196560.
    KEGGibme:BMEI0849.
    PATRICi17801877. VBIBruMel146950_3258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52030.1 .
    PIRi AC3358.
    RefSeqi NP_539766.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YHF2.
    SMRi Q8YHF2. Positions 2-540.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52030 ; AAL52030 ; BMEI0849 .
    GeneIDi 1196560.
    KEGGi bme:BMEI0849.
    PATRICi 17801877. VBIBruMel146950_3258.

    Phylogenomic databases

    HOGENOMi HOG000077515.
    KOi K01937.
    OMAi YWNVISK.
    OrthoDBi EOG6RC3NR.

    Enzyme and pathway databases

    UniPathwayi UPA00159 ; UER00277 .

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPi MF_01227. PyrG.
    InterProi IPR029062. Class_I_gatase-like.
    IPR004468. CTP_synthase.
    IPR017456. CTP_synthase_N.
    IPR017926. GATASE.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11550. PTHR11550. 1 hit.
    Pfami PF06418. CTP_synth_N. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00337. PyrG. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiPYRG_BRUME
    AccessioniPrimary (citable) accession number: Q8YHF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3