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Q8YHF0

- ENO_BRUME

UniProt

Q8YHF0 - ENO_BRUME

Protein

Enolase

Gene

eno

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

    Catalytic activityi

    2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei154 – 1541SubstrateUniRule annotation
    Binding sitei163 – 1631SubstrateUniRule annotation
    Active sitei204 – 2041Proton donorUniRule annotation
    Metal bindingi241 – 2411MagnesiumUniRule annotation
    Metal bindingi284 – 2841MagnesiumUniRule annotation
    Binding sitei284 – 2841SubstrateUniRule annotation
    Metal bindingi311 – 3111MagnesiumUniRule annotation
    Binding sitei311 – 3111SubstrateUniRule annotation
    Active sitei336 – 3361Proton acceptorUniRule annotation
    Binding sitei336 – 3361Substrate (covalent); in inhibited formUniRule annotation
    Binding sitei387 – 3871SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyaseUniRule annotation
    2-phosphoglycerate dehydrataseUniRule annotation
    Gene namesi
    Name:enoUniRule annotation
    Ordered Locus Names:BMEI0851
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
    Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell
    3. phosphopyruvate hydratase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 425425EnolasePRO_0000133853Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI0851.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YHF0.
    SMRiQ8YHF0. Positions 2-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni363 – 3664Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the enolase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000072174.
    KOiK01689.
    OMAiGHINNEL.
    OrthoDBiEOG65J589.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase.
    InterProiIPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view]
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiPF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiPS00164. ENOLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YHF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAIIDIVGR EILDSRGNPT VEVDVVLEDG SFGRAAVPSG ASTGAHEAVE    50
    LRDGGSRYLG KGVEKAVEVV NGKIFDAIAG MDAESQLLID QTLIDLDGSA 100
    NKGNLGANAI LGVSLAVAKA AAQASGLPLY RYVGGTNAHV LPVPMMNIIN 150
    GGAHADNPID FQEFMILPVG ATSIREAVRY GSEVFHTLKK RLKDAGHNTN 200
    VGDEGGFAPN LKNAQAALDF IMESIEKAGF KPGEDIALGL DCAATEFFKD 250
    GNYVYEGERK TRDPKAQAKY LAKLASDYPI VTIEDGMAED DWEGWKYLTD 300
    LIGNKCQLVG DDLFVTNSAR LRDGIRLGVA NSILVKVNQI GSLSETLDAV 350
    ETAHKAGYTA VMSHRSGETE DSTIADLAVA TNCGQIKTGS LARSDRTAKY 400
    NQLIRIEEEL DKQARYAGRS ALKLL 425
    Length:425
    Mass (Da):45,319
    Last modified:March 1, 2002 - v1
    Checksum:iA148573BD469668A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52032.1.
    PIRiAE3358.
    RefSeqiNP_539768.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52032; AAL52032; BMEI0851.
    GeneIDi1196562.
    KEGGibme:BMEI0851.
    PATRICi17801881. VBIBruMel146950_3260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52032.1 .
    PIRi AE3358.
    RefSeqi NP_539768.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YHF0.
    SMRi Q8YHF0. Positions 2-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0851.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52032 ; AAL52032 ; BMEI0851 .
    GeneIDi 1196562.
    KEGGi bme:BMEI0851.
    PATRICi 17801881. VBIBruMel146950_3260.

    Phylogenomic databases

    HOGENOMi HOG000072174.
    KOi K01689.
    OMAi GHINNEL.
    OrthoDBi EOG65J589.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00187 .

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00318. Enolase.
    InterProi IPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view ]
    PANTHERi PTHR11902. PTHR11902. 1 hit.
    Pfami PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001400. Enolase. 1 hit.
    PRINTSi PR00148. ENOLASE.
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01060. eno. 1 hit.
    PROSITEi PS00164. ENOLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiENO_BRUME
    AccessioniPrimary (citable) accession number: Q8YHF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3