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Q8YHF0

- ENO_BRUME

UniProt

Q8YHF0 - ENO_BRUME

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Protein

Enolase

Gene

eno

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei154 – 1541SubstrateUniRule annotation
Binding sitei163 – 1631SubstrateUniRule annotation
Active sitei204 – 2041Proton donorUniRule annotation
Metal bindingi241 – 2411MagnesiumUniRule annotation
Metal bindingi284 – 2841MagnesiumUniRule annotation
Binding sitei284 – 2841SubstrateUniRule annotation
Metal bindingi311 – 3111MagnesiumUniRule annotation
Binding sitei311 – 3111SubstrateUniRule annotation
Active sitei336 – 3361Proton acceptorUniRule annotation
Binding sitei336 – 3361Substrate (covalent); in inhibited formUniRule annotation
Binding sitei387 – 3871SubstrateUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:BMEI0851
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

  1. cell surface Source: UniProtKB-HAMAP
  2. extracellular region Source: UniProtKB-KW
  3. phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425EnolasePRO_0000133853Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI0851.

Structurei

3D structure databases

ProteinModelPortaliQ8YHF0.
SMRiQ8YHF0. Positions 2-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni363 – 3664Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000072174.
KOiK01689.
OMAiGHINNEL.
OrthoDBiEOG65J589.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YHF0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAIIDIVGR EILDSRGNPT VEVDVVLEDG SFGRAAVPSG ASTGAHEAVE
60 70 80 90 100
LRDGGSRYLG KGVEKAVEVV NGKIFDAIAG MDAESQLLID QTLIDLDGSA
110 120 130 140 150
NKGNLGANAI LGVSLAVAKA AAQASGLPLY RYVGGTNAHV LPVPMMNIIN
160 170 180 190 200
GGAHADNPID FQEFMILPVG ATSIREAVRY GSEVFHTLKK RLKDAGHNTN
210 220 230 240 250
VGDEGGFAPN LKNAQAALDF IMESIEKAGF KPGEDIALGL DCAATEFFKD
260 270 280 290 300
GNYVYEGERK TRDPKAQAKY LAKLASDYPI VTIEDGMAED DWEGWKYLTD
310 320 330 340 350
LIGNKCQLVG DDLFVTNSAR LRDGIRLGVA NSILVKVNQI GSLSETLDAV
360 370 380 390 400
ETAHKAGYTA VMSHRSGETE DSTIADLAVA TNCGQIKTGS LARSDRTAKY
410 420
NQLIRIEEEL DKQARYAGRS ALKLL
Length:425
Mass (Da):45,319
Last modified:March 1, 2002 - v1
Checksum:iA148573BD469668A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52032.1.
PIRiAE3358.
RefSeqiNP_539768.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52032; AAL52032; BMEI0851.
GeneIDi1196562.
KEGGibme:BMEI0851.
PATRICi17801881. VBIBruMel146950_3260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52032.1 .
PIRi AE3358.
RefSeqi NP_539768.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YHF0.
SMRi Q8YHF0. Positions 2-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0851.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL52032 ; AAL52032 ; BMEI0851 .
GeneIDi 1196562.
KEGGi bme:BMEI0851.
PATRICi 17801881. VBIBruMel146950_3260.

Phylogenomic databases

HOGENOMi HOG000072174.
KOi K01689.
OMAi GHINNEL.
OrthoDBi EOG65J589.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00187 .

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPi MF_00318. Enolase.
InterProi IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view ]
PANTHERi PTHR11902. PTHR11902. 1 hit.
Pfami PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001400. Enolase. 1 hit.
PRINTSi PR00148. ENOLASE.
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR01060. eno. 1 hit.
PROSITEi PS00164. ENOLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiENO_BRUME
AccessioniPrimary (citable) accession number: Q8YHF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3