Q8YHC6 (LON_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	BMEI0876

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.
Sequence caution	The sequence AAL52057.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	Lon protease		PRO_0000076123
Regions
	Domain	24 – 212	189	Lon
	Nucleotide binding	367 – 374	8	ATP By similarity
Sites
	Active site	689	1	By similarity
	Active site	732	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHC6 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 401E15F1FDD24E98	FASTA	812	89,896
	10 20 30 40 50 60 MTGIEQKTPV GGSETGGADG LYAVLPLRDI VVFPHMIVPL FVGREKSIRA LEEVMGVDKQ 70 80 90 100 110 120 ILLATQKNAA DDDPAPDAIY EIGTIANVLQ LLKLPDGTVK VLVEGTARAK ISKFTDREDY 130 140 150 160 170 180 HEAYAAALQE PEEDAVEIEA LARSVVSDFE NYVKLNKKIS PEVVGTASQI DDYSKLADTV 190 200 210 220 230 240 ASHLAIKIPE KQEMLSVLSV RERLEKALSF MEAEISVLQV EKRIRSRVKR QMEKTQREYY 250 260 270 280 290 300 LNEQMKAIQK ELGDSEDGRD EVAEIEERIT KTKLSKEARE KALAELKKLR SMSPMSAEAT 310 320 330 340 350 360 VVRNYLDWLL SIPWGKKSKV KQDLNFAQEV LDAEHFGLGK VKERIVEYLA VQARSTKIKG 370 380 390 400 410 420 PILCLVGPPG VGKTSLARSI AKATGREYVR MSLGGVRDEA EIRGHRRTYI GSMPGKVIQS 430 440 450 460 470 480 MKKAKKSNPL FLLDEIDKMG QDFRGDPSSA MLEVLDPKQN ATFMDHYLEV EYDLSNVMFV 490 500 510 520 530 540 TTANTMNIPG PLLDRMEIIR IAGYTEDEKL EIAKRHLLPK AIKDHALQPK EFSVTEDALR 550 560 570 580 590 600 NVIRHYTREA GVRSLEREVM TLARKAVTEI LKTKKKSVKI TDKNLSDYLG VEKFRFGQID 610 620 630 640 650 660 GEDQVGVVTG LAWTEVGGEL LTIEGVMMPG KGRMTVTGNL RDVMKESISA AASYVRSRAI 670 680 690 700 710 720 DFGIEPPLFD KRDIHVHVPE GATPKDGPSA GIAMVTAIVS VLTGIPVRKD IAMTGEVTLR 730 740 750 760 770 780 GRVLPIGGLK EKLLATLRGG IKKVLIPEEN AKDLAEIPDN VKNNLEIVPV SRVGEVLKHA 790 800 810 LVRQPEPIEW TEQENPTAVP PVEDEAGASL AH

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52057.1. Different initiation.
PIR	AF3361.
RefSeq	NP_539793.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YHC6.
SMR	Q8YHC6. Positions 502-596, 604-785.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196587.
GenomeReviews	Gene locus BMEI0876 in contig AE008917_GR.
KEGG	bme:BMEI0876.
NMPDR	fig|224914.1.peg.875.
PATRIC	17851961. VBIBruMel92729_0983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566281.
OMA	FMEGEIS.
PhylomeDB	Q8YHC6.
ProtClustDB	CLSK864999.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0876-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_BRUME

Accession

Primary (citable) accession number: Q8YHC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program