Q8YHB2 (TGT_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	BMEI0890

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135455
Sites
	Active site	94	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Binding site	95	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHB2 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9A6AAD13C86E5BD8	FASTA	377	41,466
	10 20 30 40 50 60 MTTENFGFKV LARDGAARQG EISMPRGVVR TPAFMPVGTA GTVKAMYMDQ VKELGADIIL 70 80 90 100 110 120 GNTYHLMLRP GAERVARLGG LHEFGGWKGP ILTDSGGFQV MSLAQLRKLN EHGVTFRSHI 130 140 150 160 170 180 DGKAYEMTPE RSIEIQGLLD SDIQMQLDEC VALPSPEKNT ERAMELSLRW AERCKVAFGD 190 200 210 220 230 240 QPGKAMFGIV QGGDIARLRE RSAEALKAMD LKGYSVGGLA VGEPQEVMLD MLEVVCPILP 250 260 270 280 290 300 TEKPRYLMGV GTPDDILKSV ARGIDMFDCV MPTRAGRHGL AFTRFGKVNL RNARHAEDHR 310 320 330 340 350 360 PLDPQSDCPA SRDYSRAYLH HLVKSGEALG AMLLTWNNLA YYQYLMKGIR AAIADGKFSD 370 FTAETTEGWA RGDMPAL

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52071.1.
PIR	AD3363.
RefSeq	NP_539807.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YHB2.
SMR	Q8YHB2. Positions 5-365.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196601.
GenomeReviews	Gene locus BMEI0890 in contig AE008917_GR.
KEGG	bme:BMEI0890.
NMPDR	fig|224914.1.peg.889.
PATRIC	17851991. VBIBruMel92729_0998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629929.
OMA	FMPVGTV.
PhylomeDB	Q8YHB2.
ProtClustDB	PRK00112.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0890-MONOMER.
Family and domain databases
HAMAP	MF_00168. Q_tRNA_Tgt. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KO	K00773.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BRUME

Accession

Primary (citable) accession number: Q8YHB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program