Reviewed, UniProtKB/Swiss-Prot Q8YHB2 (TGT_BRUME)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	BMEI0890

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135455
Sites
	Active site	94	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Binding site	95	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YHB2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9A6AAD13C86E5BD8	FASTA	377	41,466
	10 20 30 40 50 60 MTTENFGFKV LARDGAARQG EISMPRGVVR TPAFMPVGTA GTVKAMYMDQ VKELGADIIL 70 80 90 100 110 120 GNTYHLMLRP GAERVARLGG LHEFGGWKGP ILTDSGGFQV MSLAQLRKLN EHGVTFRSHI 130 140 150 160 170 180 DGKAYEMTPE RSIEIQGLLD SDIQMQLDEC VALPSPEKNT ERAMELSLRW AERCKVAFGD 190 200 210 220 230 240 QPGKAMFGIV QGGDIARLRE RSAEALKAMD LKGYSVGGLA VGEPQEVMLD MLEVVCPILP 250 260 270 280 290 300 TEKPRYLMGV GTPDDILKSV ARGIDMFDCV MPTRAGRHGL AFTRFGKVNL RNARHAEDHR 310 320 330 340 350 360 PLDPQSDCPA SRDYSRAYLH HLVKSGEALG AMLLTWNNLA YYQYLMKGIR AAIADGKFSD 370 FTAETTEGWA RGDMPAL

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009529 Genomic DNA. Translation: AAL52071.1.
PIR	AD3363.
RefSeq	NP_539807.1.
3D structure databases
HSSP	HSSP built from PDB template 1K4G based on UniProtKB P28720.
ModBase	Search...
Genome annotation databases
GeneID	1196601.
GenomeReviews	Gene locus BMEI0890 in contig AE008917_GR.
KEGG	bme:BMEI0890.
NMPDR	fig|224914.1.peg.889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YHB2.
OMA	Q8YHB2. ECVRLPA.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0890-MON.
BRENDA	2.4.2.29. 277959.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BRUME

Accession

Primary (citable) accession number: Q8YHB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)