Q8YH64 (XGPT_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine phosphoribosyltransferase
EC=2.4.2.22

Alternative name(s):
Xanthine-guanine phosphoribosyltransferase
Short name=XGPRT

Gene names

Name:	gpt
Ordered Locus Names:	BMEI0940

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts on guanine, xanthine and to a lesser extent hypoxanthine By similarity. HAMAP-Rule MF_01903
Catalytic activity	XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine. HAMAP-Rule MF_01903
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01903
Pathway	Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1. HAMAP-Rule MF_01903
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_01903
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_01903.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. XGPT subfamily.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	XMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: HAMAP
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Xanthine phosphoribosyltransferase HAMAP-Rule MF_01903		PRO_0000139658
Regions
	Region	41 – 42	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	102 – 106	5	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Metal binding	99	1	Magnesium By similarity
	Binding site	102	1	Xanthine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YH64 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: EB7D688B3D45A169	FASTA	170	18,821
	10 20 30 40 50 60 MSLPDKAFPV SWDQFHRDAR ALAWRIAGLD REWRAIVAIT RGGLVPAAII CRELGIRLIE 70 80 90 100 110 120 TVCIASYHDY TSQGEMQVLK GIGASLLENQ GEGVIVVDDL TDTGKTAAIV REMMPRAHFA 130 140 150 160 170 TVYAKPKGRP LIDTLRHGGL TGYLDLFPMG YGLHLSGADC WRKTRLNETK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52121.1.
PIR	AF3369.
RefSeq	NP_539857.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YH64.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0940.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52121; AAL52121; BMEI0940.
GeneID	1196651.
KEGG	bme:BMEI0940.
PATRIC	17798188. VBIBruMel146950_0955.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000226805.
KO	K00769.
OMA	TACHRRF.
ProtClustDB	PRK09177.
Enzyme and pathway databases
UniPathway	UPA00602; UER00658.
Family and domain databases
HAMAP	MF_01903. XGPRT.
InterPro	IPR000836. PRibTrfase_dom. IPR023747. Xanthine_Guanine_PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XGPT_BRUME

Accession

Primary (citable) accession number: Q8YH64

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program