Q8YH17 (SYM_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BMEI0987

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Monomer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
Sequence caution	The sequence AAL52168.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	Methionine--tRNA ligase HAMAP MF_01228		PRO_0000139212
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_01228
	Motif	300 – 304	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	303	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YH17 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: 670735824F92AF00	FASTA	515	57,998
	10 20 30 40 50 60 MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT DEHGIKMLQS 70 80 90 100 110 120 ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER HYKASQVIWQ AMVANGDIYK 130 140 150 160 170 180 GGYAGWYSVR DEAYYGEEET EVRADGVRYG PQGTPVEWVE EESYFFRLSA YQDKLLDLYE 190 200 210 220 230 240 NNPGFIMPAE RRNEIVSFVK SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA 250 260 270 280 290 300 LGYPDTTDER WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK 310 320 330 340 350 360 MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA DLANDLGNLA 370 380 390 400 410 420 QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR KAMDDQALHL ALGAIFAVVA 430 440 450 460 470 480 EANRYFAGQE PWALRKTDPA RMGTVLYVTA EVLRRVGIMV QPFIPQSAEK LLDILAAPAD 490 500 510 KRQFADVLAS PLAGGTDLPA PQPVFPRYVE ADEQN

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52168.1. Different initiation.
PIR	AE3375.
RefSeq	NP_539904.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YH17.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196698.
GenomeReviews	Gene locus BMEI0987 in contig AE008917_GR.
KEGG	bme:BMEI0987.
NMPDR	fig|224914.1.peg.986.
PATRIC	17852209. VBIBruMel92729_1104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	FDWGIKV.
PhylomeDB	Q8YH17.
ProtClustDB	PRK11893.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0987-MONOMER.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BRUME

Accession

Primary (citable) accession number: Q8YH17

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program