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Q8YGW9 (NADK_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:BMEI1036
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Sequence caution

The sequence AAL52217.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257NAD kinase HAMAP-Rule MF_00361
PRO_0000120603

Regions

Nucleotide binding46 – 472NAD By similarity
Nucleotide binding116 – 1172NAD By similarity
Nucleotide binding157 – 1626NAD By similarity

Sites

Active site461Proton acceptor By similarity
Binding site1461NAD By similarity
Binding site1541NAD; via carbonyl oxygen By similarity
Binding site2181NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YGW9 [UniParc].

Last modified October 25, 2002. Version 2.
Checksum: 4EA7A24F2404F65A

FASTA25728,261
        10         20         30         40         50         60 
MKDTSLALHF VSSGTKESLS AQKDLVERYG HVAAEDADII VALGGDGTML QALRDFMNTG 

        70         80         90        100        110        120 
KPIYGMNRGS VGFLMNEFVI ENLPERILAA QMETIRPLVM VAETDDAPPV EALAINEVSL 

       130        140        150        160        170        180 
FRQSYQAARI RITIDGKVRL QELVCDGVMV ATPAGSTAYN LSAQGPILPL EAPLLALTPV 

       190        200        210        220        230        240 
SPFRPRRWGG ALLPKHVTVR MDLLETEKRP VNAVADNNEV KSVTSVTVRE APNSQVTILF 

       250 
DKNHSWDERI LTEQFRH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52217.1. Different initiation.
PIRAF3381.
RefSeqNP_539953.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YGW9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1036.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52217; AAL52217; BMEI1036.
GeneID1196747.
KEGGbme:BMEI1036.
PATRIC17852327. VBIBruMel92729_1160.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000139847.
KOK00858.
OMAHESTVSI.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1069-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_BRUME
AccessionPrimary (citable) accession number: Q8YGW9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: July 9, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names