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Protein

NAD kinase

Gene

nadK

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461Proton acceptorUniRule annotation
Binding sitei146 – 1461NADUniRule annotation
Binding sitei154 – 1541NAD; via carbonyl oxygenUniRule annotation
Binding sitei218 – 2181NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 472NADUniRule annotation
Nucleotide bindingi116 – 1172NADUniRule annotation
Nucleotide bindingi157 – 1626NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-1069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:BMEI1036
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257NAD kinasePRO_0000120603Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI1036.

Structurei

3D structure databases

ProteinModelPortaliQ8YGW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000139847.
KOiK00858.
OMAiHESTVSI.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YGW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTSLALHF VSSGTKESLS AQKDLVERYG HVAAEDADII VALGGDGTML
60 70 80 90 100
QALRDFMNTG KPIYGMNRGS VGFLMNEFVI ENLPERILAA QMETIRPLVM
110 120 130 140 150
VAETDDAPPV EALAINEVSL FRQSYQAARI RITIDGKVRL QELVCDGVMV
160 170 180 190 200
ATPAGSTAYN LSAQGPILPL EAPLLALTPV SPFRPRRWGG ALLPKHVTVR
210 220 230 240 250
MDLLETEKRP VNAVADNNEV KSVTSVTVRE APNSQVTILF DKNHSWDERI

LTEQFRH
Length:257
Mass (Da):28,261
Last modified:October 25, 2002 - v2
Checksum:i4EA7A24F2404F65A
GO

Sequence cautioni

The sequence AAL52217.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52217.1. Different initiation.
PIRiAF3381.
RefSeqiNP_539953.1. NC_003317.1.
WP_002964061.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL52217; AAL52217; BMEI1036.
GeneIDi3787619.
KEGGibme:BMEI1036.
PATRICi17852327. VBIBruMel92729_1160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52217.1. Different initiation.
PIRiAF3381.
RefSeqiNP_539953.1. NC_003317.1.
WP_002964061.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliQ8YGW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BMEI1036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52217; AAL52217; BMEI1036.
GeneIDi3787619.
KEGGibme:BMEI1036.
PATRICi17852327. VBIBruMel92729_1160.

Phylogenomic databases

HOGENOMiHOG000139847.
KOiK00858.
OMAiHESTVSI.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-1069-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiNADK_BRUME
AccessioniPrimary (citable) accession number: Q8YGW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: May 27, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.