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Q8YGN1

- PURL_BRUME

UniProt

Q8YGN1 - PURL_BRUME

Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (05 Mar 2002)
      Previous versions | rss
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    Functioni

    Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei50 – 501UniRule annotation
    Binding sitei53 – 531ATPUniRule annotation
    Binding sitei92 – 921ATPUniRule annotation
    Metal bindingi94 – 941Magnesium 1UniRule annotation
    Active sitei96 – 961Proton acceptorUniRule annotation
    Binding sitei117 – 1171SubstrateUniRule annotation
    Metal bindingi118 – 1181Magnesium 2UniRule annotation
    Binding sitei241 – 2411SubstrateUniRule annotation
    Metal bindingi269 – 2691Magnesium 2UniRule annotation
    Binding sitei495 – 4951ATPUniRule annotation
    Binding sitei532 – 5321ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
    Metal bindingi533 – 5331Magnesium 1UniRule annotation
    Binding sitei535 – 5351SubstrateUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-1162-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
    Short name:
    FGAR amidotransferase IIUniRule annotation
    Short name:
    FGAR-AT IIUniRule annotation
    Glutamine amidotransferase PurLUniRule annotation
    Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
    Gene namesi
    Name:purLUniRule annotation
    Ordered Locus Names:BMEI1127
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 740740Phosphoribosylformylglycinamidine synthase subunit PurLPRO_0000100444Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI1127.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YGN1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni95 – 984Substrate bindingUniRule annotation
    Regioni313 – 3153Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the FGAMS family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000238227.
    KOiK01952.
    OMAiRDICGRE.
    OrthoDBiEOG6FNHHR.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    HAMAPiMF_00420. PurL_2.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8YGN1-1 [UniParc]FASTAAdd to Basket

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    MTISNTRDIT PELIEAHGLK PDEYQRILEL IGREPTFTEL GIFSAMWNEH    50
    CSYKSSKKWL RTLPTSGPRV IQGPGENAGV VDIGDGDCVV FKMESHNHPS 100
    YIEPYQGAAT GVGGILRDVF TMGARPVAAM NALRFGEPDH PKTRHLVSGV 150
    VSGVGGYGNA FGVPTVGGEV NFDKRYNGNI LVNAFAAGLA RHDGIFLSEA 200
    EGVGLPVVYL GAKTSRDGVG GATMASAEFD ESIEEKRPTV QVGDPFTEKC 250
    LLEACLELMA SGAVIAIQDM GAAGLTCSAV EMGAKGDLGI ELILDHVPVR 300
    EENMTAYEMM LSESQERMLM VLKPEKEAEA QAIFRKWGLD FAIVGKTTDD 350
    LRFRVIHQGE EVANLPIKDL GDEAPEYDRP WMEPGKHAPL PASNVPQVED 400
    YSAALLKLIG SPDLSSRRWV YEQYDTLIQG NSLQVPGGDA GVIRVEGHET 450
    KALAFSSDVT PRYCEADPFE GGKQAVAECW RNITATGAEP LASTDNLNFG 500
    NPEKPEIMGQ LVKAIEGIGE ACRALDFPIV SGNVSLYNET NGQAILPTPT 550
    IAGVGLLPDW SQMAKIGGMQ DGDTLVLLGG DGTHLGQSVY LRDLFDRADG 600
    PAPFVDLALE KRNGEFVRSA IRNGQVTACH DLSDGGLAIA VAEMAIKSGK 650
    GATLDAGDGL PHALLFGEDQ ARYVISATPE MAKLIALNAE GAGVPFRILG 700
    TVGGDRLKIS KNVDVSVADL TQAYEGWFPN FMNGELTGNN 740
    Length:740
    Mass (Da):79,148
    Last modified:March 5, 2002 - v1
    Checksum:iC59E25D61D763D78
    GO

    Sequence cautioni

    The sequence AAL52308.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52308.1. Different initiation.
    PIRiAI3392.
    RefSeqiNP_540044.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52308; AAL52308; BMEI1127.
    GeneIDi1196838.
    KEGGibme:BMEI1127.
    PATRICi17852535. VBIBruMel92729_1262.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52308.1 . Different initiation.
    PIRi AI3392.
    RefSeqi NP_540044.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YGN1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52308 ; AAL52308 ; BMEI1127 .
    GeneIDi 1196838.
    KEGGi bme:BMEI1127.
    PATRICi 17852535. VBIBruMel92729_1262.

    Phylogenomic databases

    HOGENOMi HOG000238227.
    KOi K01952.
    OMAi RDICGRE.
    OrthoDBi EOG6FNHHR.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci BMEL224914:GCJ0-1162-MONOMER.

    Miscellaneous databases

    PROi Q8YGN1.

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    HAMAPi MF_00420. PurL_2.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiPURL_BRUME
    AccessioniPrimary (citable) accession number: Q8YGN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3