Q8YGN1 (PURL_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	BMEI1127

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.
Sequence caution	The sequence AAL52308.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100444
Regions
	Nucleotide binding	107 – 118	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YGN1 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: C59E25D61D763D78	FASTA	740	79,148
	10 20 30 40 50 60 MTISNTRDIT PELIEAHGLK PDEYQRILEL IGREPTFTEL GIFSAMWNEH CSYKSSKKWL 70 80 90 100 110 120 RTLPTSGPRV IQGPGENAGV VDIGDGDCVV FKMESHNHPS YIEPYQGAAT GVGGILRDVF 130 140 150 160 170 180 TMGARPVAAM NALRFGEPDH PKTRHLVSGV VSGVGGYGNA FGVPTVGGEV NFDKRYNGNI 190 200 210 220 230 240 LVNAFAAGLA RHDGIFLSEA EGVGLPVVYL GAKTSRDGVG GATMASAEFD ESIEEKRPTV 250 260 270 280 290 300 QVGDPFTEKC LLEACLELMA SGAVIAIQDM GAAGLTCSAV EMGAKGDLGI ELILDHVPVR 310 320 330 340 350 360 EENMTAYEMM LSESQERMLM VLKPEKEAEA QAIFRKWGLD FAIVGKTTDD LRFRVIHQGE 370 380 390 400 410 420 EVANLPIKDL GDEAPEYDRP WMEPGKHAPL PASNVPQVED YSAALLKLIG SPDLSSRRWV 430 440 450 460 470 480 YEQYDTLIQG NSLQVPGGDA GVIRVEGHET KALAFSSDVT PRYCEADPFE GGKQAVAECW 490 500 510 520 530 540 RNITATGAEP LASTDNLNFG NPEKPEIMGQ LVKAIEGIGE ACRALDFPIV SGNVSLYNET 550 560 570 580 590 600 NGQAILPTPT IAGVGLLPDW SQMAKIGGMQ DGDTLVLLGG DGTHLGQSVY LRDLFDRADG 610 620 630 640 650 660 PAPFVDLALE KRNGEFVRSA IRNGQVTACH DLSDGGLAIA VAEMAIKSGK GATLDAGDGL 670 680 690 700 710 720 PHALLFGEDQ ARYVISATPE MAKLIALNAE GAGVPFRILG TVGGDRLKIS KNVDVSVADL 730 740 TQAYEGWFPN FMNGELTGNN

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52308.1. Different initiation.
PIR	AI3392.
RefSeq	NP_540044.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YGN1.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52308; AAL52308; BMEI1127.
GeneID	1196838.
KEGG	bme:BMEI1127.
PATRIC	17852535. VBIBruMel92729_1262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_BRUME

Accession

Primary (citable) accession number: Q8YGN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program