Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8YGN1 (PURL_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase 2

EC=6.3.5.3
Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II
Gene names
Name:purL
Ordered Locus Names:BMEI1127
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length740 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420

Subunit structure

Heterodimer of two subunits, PurQ and PurL By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00420.

Sequence similarities

Belongs to the FGAMS family.

Sequence caution

The sequence AAL52308.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 740740Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420
PRO_0000100444

Regions

Nucleotide binding107 – 11812ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q8YGN1 [UniParc].

Last modified March 5, 2002. Version 1.
Checksum: C59E25D61D763D78

FASTA74079,148
        10         20         30         40         50         60 
MTISNTRDIT PELIEAHGLK PDEYQRILEL IGREPTFTEL GIFSAMWNEH CSYKSSKKWL 

        70         80         90        100        110        120 
RTLPTSGPRV IQGPGENAGV VDIGDGDCVV FKMESHNHPS YIEPYQGAAT GVGGILRDVF 

       130        140        150        160        170        180 
TMGARPVAAM NALRFGEPDH PKTRHLVSGV VSGVGGYGNA FGVPTVGGEV NFDKRYNGNI 

       190        200        210        220        230        240 
LVNAFAAGLA RHDGIFLSEA EGVGLPVVYL GAKTSRDGVG GATMASAEFD ESIEEKRPTV 

       250        260        270        280        290        300 
QVGDPFTEKC LLEACLELMA SGAVIAIQDM GAAGLTCSAV EMGAKGDLGI ELILDHVPVR 

       310        320        330        340        350        360 
EENMTAYEMM LSESQERMLM VLKPEKEAEA QAIFRKWGLD FAIVGKTTDD LRFRVIHQGE 

       370        380        390        400        410        420 
EVANLPIKDL GDEAPEYDRP WMEPGKHAPL PASNVPQVED YSAALLKLIG SPDLSSRRWV 

       430        440        450        460        470        480 
YEQYDTLIQG NSLQVPGGDA GVIRVEGHET KALAFSSDVT PRYCEADPFE GGKQAVAECW 

       490        500        510        520        530        540 
RNITATGAEP LASTDNLNFG NPEKPEIMGQ LVKAIEGIGE ACRALDFPIV SGNVSLYNET 

       550        560        570        580        590        600 
NGQAILPTPT IAGVGLLPDW SQMAKIGGMQ DGDTLVLLGG DGTHLGQSVY LRDLFDRADG 

       610        620        630        640        650        660 
PAPFVDLALE KRNGEFVRSA IRNGQVTACH DLSDGGLAIA VAEMAIKSGK GATLDAGDGL 

       670        680        690        700        710        720 
PHALLFGEDQ ARYVISATPE MAKLIALNAE GAGVPFRILG TVGGDRLKIS KNVDVSVADL 

       730        740 
TQAYEGWFPN FMNGELTGNN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52308.1. Different initiation.
PIRAI3392.
RefSeqNP_540044.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YGN1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52308; AAL52308; BMEI1127.
GeneID1196838.
KEGGbme:BMEI1127.
PATRIC17852535. VBIBruMel92729_1262.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000238227.
KOK01952.
OMASQERMLF.
OrthoDBEOG6FNHHR.
ProtClustDBPRK01213.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1162-MONOMER.
UniPathwayUPA00074; UER00128.

Family and domain databases

Gene3D3.30.1330.10. 2 hits.
HAMAPMF_00420. PurL_2.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetSearch...

Other

PROQ8YGN1.

Entry information

Entry namePURL_BRUME
AccessionPrimary (citable) accession number: Q8YGN1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names