Q8YGL8 (SYP_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BMEI1140

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01570
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01570
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01570.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.
Sequence caution	The sequence AAL52321.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Proline--tRNA ligase HAMAP-Rule MF_01570		PRO_0000248894

Sequences

	Sequence		Length	Mass (Da)
	Q8YGL8 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 4E9AD5F6F4AD5AB8	FASTA	442	49,477
	10 20 30 40 50 60 MRLSRYFLPI LKENPKEAEI VSHRLMLRSG MIRQQSAGIY SWLPIGLKVL NKVCTIIREE 70 80 90 100 110 120 QNRAGANEIL MPTIQSADLW RESGRYDAYG KEMLRIQDRQ KREMLFGPTN EEMVTDIFRS 130 140 150 160 170 180 YVRSYKDLPL NLYHIQWKFR DEVRPRFGVM RSREFLMKDA YSFDLDYEGA KMAYYRMFVS 190 200 210 220 230 240 YLRTFARVGL QAIPMRADTG PIGGDLSHEF IILAETGESQ VYCDRAYLDL AVPGADTDFR 250 260 270 280 290 300 NDAQLTDTVT RWTTPYAATD EMHDEADWAK VKPESQVSAR GIEVGHIFHF GTKYSEPMGA 310 320 330 340 350 360 KVQGPDGKEH LVSMGSYGIG PSRLVAAAIE ASHDDAGIIW PKTIAPFGAG IVNMKPGDEG 370 380 390 400 410 420 CDGVSEKLYE ALTNAGVDPL LDDKDERPGA KFATIDLIGL PTQVIVGPRG VAAGEVEVKD 430 440 RKTGERQSLG IEAAINMLTA QA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52321.1. Different initiation.
PIR	AF3394.
RefSeq	NP_540057.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YGL8.
SMR	Q8YGL8. Positions 1-439.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52321; AAL52321; BMEI1140.
GeneID	1196851.
KEGG	bme:BMEI1140.
PATRIC	17852561. VBIBruMel92729_1275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076894.
KO	K01881.
OMA	MVGANET.
ProtClustDB	PRK12325.
Enzyme and pathway databases
BioCyc	BMEL224914:GCJ0-1175-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
HAMAP	MF_01570. Pro_tRNA_synth_type2.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023716. Prolyl-tRNA_ligase_IIa_type2. [Graphical view]
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BRUME

Accession

Primary (citable) accession number: Q8YGL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	May 29, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program