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Q8YGL8

- SYP_BRUME

UniProt

Q8YGL8 - SYP_BRUME

Protein

Proline--tRNA ligase

Gene

proS

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-1175-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Short name:
    ProRSUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:BMEI1140
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Proline--tRNA ligasePRO_0000248894Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI1140.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YGL8.
    SMRiQ8YGL8. Positions 1-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000076894.
    KOiK01881.
    OMAiYSFHEDT.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    HAMAPiMF_01570. Pro_tRNA_synth_type2.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023716. Prolyl-tRNA_ligase_IIa_type2.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YGL8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLSRYFLPI LKENPKEAEI VSHRLMLRSG MIRQQSAGIY SWLPIGLKVL    50
    NKVCTIIREE QNRAGANEIL MPTIQSADLW RESGRYDAYG KEMLRIQDRQ 100
    KREMLFGPTN EEMVTDIFRS YVRSYKDLPL NLYHIQWKFR DEVRPRFGVM 150
    RSREFLMKDA YSFDLDYEGA KMAYYRMFVS YLRTFARVGL QAIPMRADTG 200
    PIGGDLSHEF IILAETGESQ VYCDRAYLDL AVPGADTDFR NDAQLTDTVT 250
    RWTTPYAATD EMHDEADWAK VKPESQVSAR GIEVGHIFHF GTKYSEPMGA 300
    KVQGPDGKEH LVSMGSYGIG PSRLVAAAIE ASHDDAGIIW PKTIAPFGAG 350
    IVNMKPGDEG CDGVSEKLYE ALTNAGVDPL LDDKDERPGA KFATIDLIGL 400
    PTQVIVGPRG VAAGEVEVKD RKTGERQSLG IEAAINMLTA QA 442
    Length:442
    Mass (Da):49,477
    Last modified:September 5, 2006 - v2
    Checksum:i4E9AD5F6F4AD5AB8
    GO

    Sequence cautioni

    The sequence AAL52321.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52321.1. Different initiation.
    PIRiAF3394.
    RefSeqiNP_540057.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52321; AAL52321; BMEI1140.
    GeneIDi1196851.
    KEGGibme:BMEI1140.
    PATRICi17852561. VBIBruMel92729_1275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52321.1 . Different initiation.
    PIRi AF3394.
    RefSeqi NP_540057.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YGL8.
    SMRi Q8YGL8. Positions 1-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1140.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52321 ; AAL52321 ; BMEI1140 .
    GeneIDi 1196851.
    KEGGi bme:BMEI1140.
    PATRICi 17852561. VBIBruMel92729_1275.

    Phylogenomic databases

    HOGENOMi HOG000076894.
    KOi K01881.
    OMAi YSFHEDT.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci BMEL224914:GCJ0-1175-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    HAMAPi MF_01570. Pro_tRNA_synth_type2.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023716. Prolyl-tRNA_ligase_IIa_type2.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiSYP_BRUME
    AccessioniPrimary (citable) accession number: Q8YGL8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3