Q8YGK3 (NUOD_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit D
NDH-1 subunit D

Gene names

Name:	nuoD
Ordered Locus Names:	BMEI1155

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01358
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01358
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01358.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	NADH-quinone oxidoreductase subunit D HAMAP-Rule MF_01358		PRO_0000357786

Sequences

	Sequence		Length	Mass (Da)
	Q8YGK3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AF24D18216459F0D	FASTA	396	45,011
	10 20 30 40 50 60 MAETQVRNFN INFGPQHPAA HGVLRLVLEL DGEVVERVDP HIGLLHRGTE KLMEAKTYLQ 70 80 90 100 110 120 AVPYLDRLDY VAPMNQEHAY ALAVERLLDI EVPKRGQLIR VLYSEIGRIL NHLLNVTTQA 130 140 150 160 170 180 MDVGALTPPL WGFEEREKLM VFYERACGAR MHAAYFRPGG VHQDLPDQLI EDIGKWIDPF 190 200 210 220 230 240 FTTLKNLDDL ITPNRIFKQR NVDIGVVKLE DAWAWGFSGV MVRGSGAAWD LRKSQPYECY 250 260 270 280 290 300 SEMEFDIPVG KNGDCYDRYL IRMEEMRQSV RIMRQCVDLL LGKERVGPVS NTDHKIVPPK 310 320 330 340 350 360 RGEMKRSMEA LIHHFKLYTE GYHVPAGEVY AAVEAPKGEF GVYLVSDGSN KPYRCKLRAP 370 380 390 GFAHLQAMDF LCRGHMLADV SAILGSLDIV FGEVDR

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52336.1.
PIR	AE3396.
RefSeq	NP_540072.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YGK3.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1155.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52336; AAL52336; BMEI1155.
GeneID	1196866.
KEGG	bme:BMEI1155.
PATRIC	17797708. VBIBruMel146950_0669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000228264.
KO	K00333.
OMA	QSNRIIK.
ProtClustDB	PRK06075.
Family and domain databases
HAMAP	MF_01358. NDH1_NuoD.
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOD_BRUME

Accession

Primary (citable) accession number: Q8YGK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program