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Q8YGK1

- NUOB_BRUME

UniProt

Q8YGK1 - NUOB_BRUME

Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.By similarity

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi72 – 721Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi73 – 731Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi167 – 1671Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: UniProtKB-HAMAP
    3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    4. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit BUniRule annotation
    NDH-1 subunit BUniRule annotation
    Gene namesi
    Name:nuoBUniRule annotation
    Ordered Locus Names:BMEI1157
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 193193NADH-quinone oxidoreductase subunit BPRO_0000358359Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI1157.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YGK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 20 kDa subunit family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000228249.
    KOiK00331.
    OrthoDBiEOG62K20C.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    HAMAPiMF_01356. NDH1_NuoB.
    InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view]
    PfamiPF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
    PROSITEiPS01150. COMPLEX1_20K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YGK1-1 [UniParc]FASTAAdd to Basket

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    MGLTGTNTTL VAPQPKGILD PRTGKPVGSD DAFFNDLNGE LSDKGFIVTS    50
    ADALITWART GSLMWMTFGL ACCAVEMMHI SMPRYDAERF GIAPRASPRQ 100
    SDVMIVAGTL TNKMAPALRK VYDQMPEPRY VISMGSCANG GGYYHYSYSV 150
    VRGCDRVVPV DIYVPGCPPT AEALLYGILL LQKKIRRTGT IER 193
    Length:193
    Mass (Da):21,032
    Last modified:December 16, 2008 - v2
    Checksum:i07E2DF39F0E5DF32
    GO

    Sequence cautioni

    The sequence AAL52338.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52338.1. Different initiation.
    PIRiAG3396.
    RefSeqiNP_540074.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52338; AAL52338; BMEI1157.
    GeneIDi1196868.
    KEGGibme:BMEI1157.
    PATRICi17797704. VBIBruMel146950_0667.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52338.1 . Different initiation.
    PIRi AG3396.
    RefSeqi NP_540074.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YGK1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52338 ; AAL52338 ; BMEI1157 .
    GeneIDi 1196868.
    KEGGi bme:BMEI1157.
    PATRICi 17797704. VBIBruMel146950_0667.

    Phylogenomic databases

    HOGENOMi HOG000228249.
    KOi K00331.
    OrthoDBi EOG62K20C.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    HAMAPi MF_01356. NDH1_NuoB.
    InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view ]
    Pfami PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
    PROSITEi PS01150. COMPLEX1_20K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiNUOB_BRUME
    AccessioniPrimary (citable) accession number: Q8YGK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3