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Q8YGK1 (NUOB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit B

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit B
NDH-1 subunit B
Gene names
Name:nuoB
Ordered Locus Names:BMEI1157
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01356

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01356

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01356

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex By similarity.

Subcellular location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01356.

Sequence similarities

Belongs to the complex I 20 kDa subunit family.

Sequence caution

The sequence AAL52338.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
NAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

NADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193NADH-quinone oxidoreductase subunit B HAMAP-Rule MF_01356
PRO_0000358359

Sites

Metal binding721Iron-sulfur (4Fe-4S) Potential
Metal binding731Iron-sulfur (4Fe-4S) Potential
Metal binding1371Iron-sulfur (4Fe-4S) Potential
Metal binding1671Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8YGK1 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 07E2DF39F0E5DF32

FASTA19321,032
        10         20         30         40         50         60 
MGLTGTNTTL VAPQPKGILD PRTGKPVGSD DAFFNDLNGE LSDKGFIVTS ADALITWART 

        70         80         90        100        110        120 
GSLMWMTFGL ACCAVEMMHI SMPRYDAERF GIAPRASPRQ SDVMIVAGTL TNKMAPALRK 

       130        140        150        160        170        180 
VYDQMPEPRY VISMGSCANG GGYYHYSYSV VRGCDRVVPV DIYVPGCPPT AEALLYGILL 

       190 
LQKKIRRTGT IER 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52338.1. Different initiation.
PIRAG3396.
RefSeqNP_540074.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YGK1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1157.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52338; AAL52338; BMEI1157.
GeneID1196868.
KEGGbme:BMEI1157.
PATRIC17797704. VBIBruMel146950_0667.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000228249.
KOK00331.
OrthoDBEOG62K20C.
ProtClustDBPRK06411.

Family and domain databases

Gene3D3.40.50.700. 1 hit.
HAMAPMF_01356. NDH1_NuoB.
InterProIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsTIGR01957. nuoB_fam. 1 hit.
PROSITEPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOB_BRUME
AccessionPrimary (citable) accession number: Q8YGK1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: February 19, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names