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Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi73 – 731Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi167 – 1671Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit BUniRule annotation
NDH-1 subunit BUniRule annotation
Gene namesi
Name:nuoBUniRule annotation
Ordered Locus Names:BMEI1157
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193NADH-quinone oxidoreductase subunit BPRO_0000358359Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_1052.

Structurei

3D structure databases

ProteinModelPortaliQ8YGK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228249.
KOiK00331.
OrthoDBiEOG62K20C.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YGK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTGTNTTL VAPQPKGILD PRTGKPVGSD DAFFNDLNGE LSDKGFIVTS
60 70 80 90 100
ADALITWART GSLMWMTFGL ACCAVEMMHI SMPRYDAERF GIAPRASPRQ
110 120 130 140 150
SDVMIVAGTL TNKMAPALRK VYDQMPEPRY VISMGSCANG GGYYHYSYSV
160 170 180 190
VRGCDRVVPV DIYVPGCPPT AEALLYGILL LQKKIRRTGT IER
Length:193
Mass (Da):21,032
Last modified:December 16, 2008 - v2
Checksum:i07E2DF39F0E5DF32
GO

Sequence cautioni

The sequence AAL52338.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52338.1. Different initiation.
PIRiAG3396.
RefSeqiNP_540074.1. NC_003317.1.
WP_002967573.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL52338; AAL52338; BMEI1157.
KEGGibme:BMEI1157.
PATRICi17797704. VBIBruMel146950_0667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52338.1. Different initiation.
PIRiAG3396.
RefSeqiNP_540074.1. NC_003317.1.
WP_002967573.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliQ8YGK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_1052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52338; AAL52338; BMEI1157.
KEGGibme:BMEI1157.
PATRICi17797704. VBIBruMel146950_0667.

Phylogenomic databases

HOGENOMiHOG000228249.
KOiK00331.
OrthoDBiEOG62K20C.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiNUOB_BRUME
AccessioniPrimary (citable) accession number: Q8YGK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: June 24, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.