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Q8YGH4 (HPPA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Gene names
Name:hppA
Ordered Locus Names:BMEI1185
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01129

Cofactor

Magnesium By similarity. HAMAP MF_01129

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Sequence caution

The sequence AAL52366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129
PRO_0000217011

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane83 – 10321Helical; Potential
Transmembrane112 – 13221Helical; Potential
Transmembrane133 – 15321Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane235 – 25521Helical; Potential
Transmembrane265 – 28521Helical; Potential
Transmembrane300 – 32021Helical; Potential
Transmembrane335 – 35521Helical; Potential
Transmembrane385 – 40521Helical; Potential
Transmembrane413 – 43321Helical; Potential
Transmembrane472 – 49221Helical; Potential
Transmembrane524 – 54421Helical; Potential
Transmembrane593 – 61321Helical; Potential
Transmembrane620 – 64021Helical; Potential
Transmembrane695 – 71521Helical; Potential

Sites

Site4711Determinant of potassium independence By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YGH4 [UniParc].

Last modified July 25, 2003. Version 2.
Checksum: 41312F2B1B2350C6

FASTA71873,341
        10         20         30         40         50         60 
MQMGMAVLVL VIACGVLSVL FAIWAIRSVL AADQGTQRMQ EIAEAIREGA SAYLTRQYST 

        70         80         90        100        110        120 
IAIVGIVVFL LAWYLLSLNA AMGFLIGAVL SGVTGFIGMH VSVRANVRTA QAASLSLAGG 

       130        140        150        160        170        180 
LELAFKSGAI TGLLVAGLAL LGVSVYYFVL TVWLGYAPAD RTVIDSLVSL GFGASLISIF 

       190        200        210        220        230        240 
ARLGGGIFTK GADVGGDLVG KVEAGIPEDD PRNPATIADN VGDNVGDCAG MAADLFETYA 

       250        260        270        280        290        300 
VTVVATMVLG AIFFHGSDAL TNVMLYPLMI CGACVITSIA GTFFVKLGVN GSIMGALYKG 

       310        320        330        340        350        360 
LIATGLLSIV GLGVANTLTV GWGEIGTVAG KSITGTNLFV CGLIGLIVTG LIVVITEYYT 

       370        380        390        400        410        420 
GTNKRPVNSI AQASVTGHGT NVIQGLAVSL ESTALPAIVI VGGIISTYQL AGLFGTAIAV 

       430        440        450        460        470        480 
TAMLGIAGMI VALDAFGPVT DNAGGIAEMA GLDPEVRKAT DALDAVGNTT KAVTKGYAIG 

       490        500        510        520        530        540 
SAGLGALVLF AAYSNDLAYF AANGQIYPYF ADMGPVSFDL SNPYVVAGLI FGGLIPYLFG 

       550        560        570        580        590        600 
GMAMTAVGRA GGAVVQEVRR QFREKPGIMT GKERPDYARA VDLLTKAAIR EMIIPSLLPV 

       610        620        630        640        650        660 
LAPIVVYFGV LLISGSKAAA FAALGASLLG VIINGLFVAI SMTSGGGAWD NAKKSFEDGF 

       670        680        690        700        710 
TDADGVKHMK GSEAHKASVT GDTVGDPYKD TAGPAVNPAI KITNIVALLL LAVLAHMA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE008917 Genomic DNA. Translation: AAL52366.1. Different initiation.
PIRAC3400.
RefSeqNP_540102.2. NC_003317.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1196896.
GenomeReviewsGene locus BMEI1185 in contig AE008917_GR.
KEGGbme:BMEI1185.
PATRIC17797638. VBIBruMel146950_0637.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG593668.
OMAQYMTIAI.
PhylomeDBQ8YGH4.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycBMEL224914:BMEI1185-MONOMER.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA_BRUME
AccessionPrimary (citable) accession number: Q8YGH4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

SIMILARITY comments

Index of protein domains and families

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