Q8YGH2 (RISB1_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase 1
Short name=DMRL synthase 1
Short name=Lumazine synthase 1
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase 1 beta chain

Gene names

Name:	ribH1
Ordered Locus Names:	BMEI1187

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

6,7-dimethyl-8-ribityllumazine synthase 1 HAMAP MF_00178

PRO_0000134726

Secondary structure

1

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157

Helix Strand Turn

Details...

Beta strand

14 – 20

7

Helix

24 – 41

18

Beta strand

44 – 52

9

Helix

53 – 55

3

Helix

56 – 67

12

Turn

68 – 70

3

Beta strand

75 – 84

10

Helix

90 – 108

19

Beta strand

112 – 122

11

Helix

123 – 130

8

Turn

132 – 135

4

Helix

137 – 154

18

Sequences

	Sequence		Length	Mass (Da)
	Q8YGH2 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 657DE105E3241275	FASTA	157	16,810
	10 20 30 40 50 60 MEFLMSKHEA DAPHLLIVEA RFYDDLADAL LDGAKAALDE AGATYDVVTV PGALEIPATI 70 80 90 100 110 120 SFALDGADNG GTEYDGFVAL GTVIRGETYH FDIVSNESCR ALTDLSVEES IAIGNGILTV 130 140 150 ENEEQAWVRA RREDKDKGGF AARAALTMIG LRKKFGA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE008917 Genomic DNA. Translation: AAL52368.1.

PIR

AE3400.

RefSeq

NP_540104.1. NC_003317.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O6H	X-ray	2.70	A/B/C/D/E	1-157	[»]

ProteinModelPortal

Q8YGH2.

SMR

Q8YGH2. Positions 11-157.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1196898.

GenomeReviews

Gene locus BMEI1187 in contig AE008917_GR.

KEGG

bme:BMEI1187.

NMPDR

fig|224914.1.peg.1186.

PATRIC

17797634. VBIBruMel146950_0635.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG311126.

OMA

SSRALMD.

PhylomeDB

Q8YGH2.

ProtClustDB

PRK00061.

Enzyme and pathway databases

BioCyc

BMEL224914:BMEI1187-MONOMER.

Family and domain databases

HAMAP

MF_00178. Lumazine_synth.
[Tree]

InterPro

IPR002180. DMRL_synthase.
[Graphical view]

Gene3D

G3DSA:3.40.50.960. DMRL_synthase. 1 hit.

KO

K00794.

PANTHER

PTHR21058. DMRL_synthase. 1 hit.

Pfam

PF00885. DMRL_synthase. 1 hit.
[Graphical view]

SUPFAM

SSF52121. DMRL_synthase. 1 hit.

TIGRFAMs

TIGR00114. Lumazine-synth. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RISB1_BRUME

Accession

Primary (citable) accession number: Q8YGH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program