Serine hydroxymethyltransferase - Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

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Q8YGG7 (GLYA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	BMEI1192/BMEI1191

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H₂O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051 Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.
Sequence caution	The sequence AAL52372.1 differs from that shown. Reason: Frameshift at position 187.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine biosynthetic process from serine Inferred from electronic annotation. Source: HAMAP tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

438

Serine hydroxymethyltransferase HAMAP-Rule MF_00051

PRO_0000113545

Regions

Region

3

Substrate binding By similarity

Sites

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Substrate; via carbonyl oxygen By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity

Binding site

1

Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue

1

N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8YGG7 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: EE95968DB1AD762F
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438

46,995

        10         20         30         40         50         60 
MSQANAATKA SSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI VSRAVLEAQG 

        70         80         90        100        110        120 
SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA EFANVQPNSG SQMNQAVFLA 

       130        140        150        160        170        180 
LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK WFNVVSYGVR KDDHLLDMDE VARLARENKP 

       190        200        210        220        230        240 
KLILAGGTAY SRIWDWKRFR EIADEVGACL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT 

       250        260        270        280        290        300 
HKSLRGPRGG MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK 

       310        320        330        340        350        360 
NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR ANITCNKNGI 

       370        380        390        400        410        420 
PFDPEKPFVA SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV LDGLKVANSD EGNAAVEQAV 

       430 
KEKVIALTGR FPMYGYQG

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis."
DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E.

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Overbeek R.
Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52373.1. Frameshift. AE008917 Genomic DNA. Translation: AAL52372.1. Frameshift.
PIR	AB3401. AI3400.
RefSeq	NP_540108.1. NC_003317.1. NP_540109.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YGG7.
SMR	Q8YGG7. Positions 24-428.
ModBase	Search...
Proteomic databases
PRIDE	Q8YGG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52372; AAL52372; BMEI1191. AAL52373; AAL52373; BMEI1192.
GeneID	1196902. 1196903.
KEGG	bme:BMEI1191. bme:BMEI1192.
PATRIC	17852683. VBIBruMel92729_1333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00600.
OMA	IFGAIGK.
Enzyme and pathway databases
UniPathway	UPA00193. UPA00288; UER01023.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_00051. SHMT.
InterPro	IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
PANTHER	PTHR11680. PTHR11680. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_BRUME

Accession

Primary (citable) accession number: Q8YGG7
Secondary accession number(s): Q8YGG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents