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Q8YGG7

- GLYA_BRUME

UniProt

Q8YGG7 - GLYA_BRUME

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Protein
Serine hydroxymethyltransferase
Gene
glyA, BMEI1192/BMEI1191
Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471Pyridoxal phosphate By similarity
Binding sitei67 – 671Pyridoxal phosphate By similarity
Binding sitei69 – 691Substrate By similarity
Binding sitei76 – 761Substrate By similarity
Binding sitei77 – 771Pyridoxal phosphate By similarity
Binding sitei111 – 1111Pyridoxal phosphate By similarity
Binding sitei133 – 1331Substrate; via carbonyl oxygen By similarity
Binding sitei188 – 1881Pyridoxal phosphate By similarity
Binding sitei216 – 2161Pyridoxal phosphate By similarity
Binding sitei241 – 2411Pyridoxal phosphate By similarity
Binding sitei248 – 2481Pyridoxal phosphate By similarity
Binding sitei274 – 2741Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei374 – 3741Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-1229-MONOMER.
BMEL224914:GCJ0-1230-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase (EC:2.1.2.1)
Short name:
SHMT
Short name:
Serine methylase
Gene namesi
Name:glyA
Ordered Locus Names:BMEI1192/BMEI1191
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Serine hydroxymethyltransferaseUniRule annotation
PRO_0000113545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PRIDEiQ8YGG7.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8YGG7.
SMRiQ8YGG7. Positions 24-428.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1393Substrate binding By similarity

Sequence similaritiesi

Belongs to the SHMT family.

Phylogenomic databases

KOiK00600.
OMAiDDQRIDY.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YGG7-1 [UniParc]FASTAAdd to Basket

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MSQANAATKA SSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI    50
VSRAVLEAQG SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA 100
EFANVQPNSG SQMNQAVFLA LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK 150
WFNVVSYGVR KDDHLLDMDE VARLARENKP KLILAGGTAY SRIWDWKRFR 200
EIADEVGACL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT HKSLRGPRGG 250
MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK 300
NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR 350
ANITCNKNGI PFDPEKPFVA SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV 400
LDGLKVANSD EGNAAVEQAV KEKVIALTGR FPMYGYQG 438
Length:438
Mass (Da):46,995
Last modified:November 7, 2003 - v2
Checksum:iEE95968DB1AD762F
GO

Sequence cautioni

The sequence AAL52372.1 differs from that shown. Reason: Frameshift at position 187.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52373.1. Frameshift.
AE008917 Genomic DNA. Translation: AAL52372.1. Frameshift.
PIRiAB3401.
AI3400.
RefSeqiNP_540108.1. NC_003317.1.
NP_540109.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52372; AAL52372; BMEI1191.
AAL52373; AAL52373; BMEI1192.
GeneIDi1196902.
1196903.
KEGGibme:BMEI1191.
bme:BMEI1192.
PATRICi17852683. VBIBruMel92729_1333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL52373.1 . Frameshift.
AE008917 Genomic DNA. Translation: AAL52372.1 . Frameshift.
PIRi AB3401.
AI3400.
RefSeqi NP_540108.1. NC_003317.1.
NP_540109.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YGG7.
SMRi Q8YGG7. Positions 24-428.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q8YGG7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL52372 ; AAL52372 ; BMEI1191 .
AAL52373 ; AAL52373 ; BMEI1192 .
GeneIDi 1196902.
1196903.
KEGGi bme:BMEI1191.
bme:BMEI1192.
PATRICi 17852683. VBIBruMel92729_1333.

Phylogenomic databases

KOi K00600.
OMAi DDQRIDY.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci BMEL224914:GCJ0-1229-MONOMER.
BMEL224914:GCJ0-1230-MONOMER.

Miscellaneous databases

PROi Q8YGG7.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiGLYA_BRUME
AccessioniPrimary (citable) accession number: Q8YGG7
Secondary accession number(s): Q8YGG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: May 14, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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