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Q8YGG7 (GLYA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase

Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:BMEI1192/BMEI1191
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence caution

The sequence AAL52372.1 differs from that shown. Reason: Frameshift at position 187.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
One-carbon metabolism
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine biosynthetic process from serine

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycine hydroxymethyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Serine hydroxymethyltransferase HAMAP-Rule MF_00051
PRO_0000113545

Regions

Region137 – 1393Substrate binding By similarity

Sites

Binding site471Pyridoxal phosphate By similarity
Binding site671Pyridoxal phosphate By similarity
Binding site691Substrate By similarity
Binding site761Substrate By similarity
Binding site771Pyridoxal phosphate By similarity
Binding site1111Pyridoxal phosphate By similarity
Binding site1331Substrate; via carbonyl oxygen By similarity
Binding site1881Pyridoxal phosphate By similarity
Binding site2161Pyridoxal phosphate By similarity
Binding site2411Pyridoxal phosphate By similarity
Binding site2481Pyridoxal phosphate By similarity
Binding site2741Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3741Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2421N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YGG7 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: EE95968DB1AD762F

FASTA43846,995
        10         20         30         40         50         60 
MSQANAATKA SSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI VSRAVLEAQG 

        70         80         90        100        110        120 
SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA EFANVQPNSG SQMNQAVFLA 

       130        140        150        160        170        180 
LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK WFNVVSYGVR KDDHLLDMDE VARLARENKP 

       190        200        210        220        230        240 
KLILAGGTAY SRIWDWKRFR EIADEVGACL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT 

       250        260        270        280        290        300 
HKSLRGPRGG MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK 

       310        320        330        340        350        360 
NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR ANITCNKNGI 

       370        380        390        400        410        420 
PFDPEKPFVA SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV LDGLKVANSD EGNAAVEQAV 

       430 
KEKVIALTGR FPMYGYQG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52373.1. Frameshift.
AE008917 Genomic DNA. Translation: AAL52372.1. Frameshift.
PIRAB3401.
AI3400.
RefSeqNP_540108.1. NC_003317.1.
NP_540109.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YGG7.
SMRQ8YGG7. Positions 24-428.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8YGG7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52372; AAL52372; BMEI1191.
AAL52373; AAL52373; BMEI1192.
GeneID1196902.
1196903.
KEGGbme:BMEI1191.
bme:BMEI1192.
PATRIC17852683. VBIBruMel92729_1333.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00600.
OMADDQRIDY.
OrthoDBEOG6Z0QB2.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1229-MONOMER.
BMEL224914:GCJ0-1230-MONOMER.
UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8YGG7.

Entry information

Entry nameGLYA_BRUME
AccessionPrimary (citable) accession number: Q8YGG7
Secondary accession number(s): Q8YGG8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: May 14, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names