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Q8YGG7

- GLYA_BRUME

UniProt

Q8YGG7 - GLYA_BRUME

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei47 – 471Pyridoxal phosphateUniRule annotation
    Binding sitei67 – 671Pyridoxal phosphateUniRule annotation
    Binding sitei69 – 691SubstrateUniRule annotation
    Binding sitei76 – 761SubstrateUniRule annotation
    Binding sitei77 – 771Pyridoxal phosphateUniRule annotation
    Binding sitei111 – 1111Pyridoxal phosphateUniRule annotation
    Binding sitei133 – 1331Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei188 – 1881Pyridoxal phosphateUniRule annotation
    Binding sitei216 – 2161Pyridoxal phosphateUniRule annotation
    Binding sitei241 – 2411Pyridoxal phosphateUniRule annotation
    Binding sitei248 – 2481Pyridoxal phosphateUniRule annotation
    Binding sitei274 – 2741Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei374 – 3741Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-1229-MONOMER.
    BMEL224914:GCJ0-1230-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:BMEI1192/BMEI1191
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 438438Serine hydroxymethyltransferasePRO_0000113545Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei242 – 2421N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PRIDEiQ8YGG7.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YGG7.
    SMRiQ8YGG7. Positions 24-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1393Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    KOiK00600.
    OMAiDDQRIDY.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YGG7-1 [UniParc]FASTAAdd to Basket

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    MSQANAATKA SSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI    50
    VSRAVLEAQG SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA 100
    EFANVQPNSG SQMNQAVFLA LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK 150
    WFNVVSYGVR KDDHLLDMDE VARLARENKP KLILAGGTAY SRIWDWKRFR 200
    EIADEVGACL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT HKSLRGPRGG 250
    MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK 300
    NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR 350
    ANITCNKNGI PFDPEKPFVA SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV 400
    LDGLKVANSD EGNAAVEQAV KEKVIALTGR FPMYGYQG 438
    Length:438
    Mass (Da):46,995
    Last modified:November 7, 2003 - v2
    Checksum:iEE95968DB1AD762F
    GO

    Sequence cautioni

    The sequence AAL52372.1 differs from that shown. Reason: Frameshift at position 187.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52373.1. Frameshift.
    AE008917 Genomic DNA. Translation: AAL52372.1. Frameshift.
    PIRiAB3401.
    AI3400.
    RefSeqiNP_540108.1. NC_003317.1.
    NP_540109.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52372; AAL52372; BMEI1191.
    AAL52373; AAL52373; BMEI1192.
    GeneIDi1196902.
    1196903.
    KEGGibme:BMEI1191.
    bme:BMEI1192.
    PATRICi17852683. VBIBruMel92729_1333.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52373.1 . Frameshift.
    AE008917 Genomic DNA. Translation: AAL52372.1 . Frameshift.
    PIRi AB3401.
    AI3400.
    RefSeqi NP_540108.1. NC_003317.1.
    NP_540109.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YGG7.
    SMRi Q8YGG7. Positions 24-428.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8YGG7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52372 ; AAL52372 ; BMEI1191 .
    AAL52373 ; AAL52373 ; BMEI1192 .
    GeneIDi 1196902.
    1196903.
    KEGGi bme:BMEI1191.
    bme:BMEI1192.
    PATRICi 17852683. VBIBruMel92729_1333.

    Phylogenomic databases

    KOi K00600.
    OMAi DDQRIDY.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci BMEL224914:GCJ0-1229-MONOMER.
    BMEL224914:GCJ0-1230-MONOMER.

    Miscellaneous databases

    PROi Q8YGG7.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiGLYA_BRUME
    AccessioniPrimary (citable) accession number: Q8YGG7
    Secondary accession number(s): Q8YGG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3