Reviewed, UniProtKB/Swiss-Prot Q8YG99 (AMPA_BRUME)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BMEI1261

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165728
Sites
	Active site	263	1	Potential
	Active site	337	1	Potential
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	256	1	Manganese 1 By similarity
	Metal binding	256	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	333	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YG99-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9326347A61BFCCFF	FASTA	485	51,868
	10 20 30 40 50 60 MKYRKRGWPS CWWPRAAVLP MRRQGVGGAE KIVRIADISG FTGALGKTAE AIETTPAGVE 70 80 90 100 110 120 KIVLVGVGEP GKLGNDDWLK IGGAAFSQIG NAERVTLTLA LPETTIAGDE AADVALGMVL 130 140 150 160 170 180 RSYKFDRYKT RKSEENGEPK HAAKITVCVA DTHSARKAFE VAEAVADGVI QARNLVNEPA 190 200 210 220 230 240 NILGPVEFAE EAEKLEKLGV KVEVLGEKEL KKLGMGALLG VAQGSVRPPR LVVMEWHGAK 250 260 270 280 290 300 GKEKPIAFVG KGVVFDTGGI SIKPAANMED MKGDMGGAAA VTGLMRALAG RKAKVNAIGV 310 320 330 340 350 360 IGLVENMPDG NAQRPGDIVT SMSGQTIEVI NTDAEGRLVL ADALHYTNDR FKPRFIINLA 370 380 390 400 410 420 TLTGAVMVAL GQYHAGLFSN DDELADQLYD AGQSTGEKLW RLPLGTEYDK MIDSKFADMK 430 440 450 460 470 480 NSAGRYGGSI TAAQFLKRFV GETPWAHLDV AGTAMGSPAN EYNQSWASGF GVRLLDRLVR DQFES

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL52442.1.
PIR	AG3409.
RefSeq	NP_540178.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYT based on UniProtKB P11648.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	1196972.
GenomeReviews	Gene locus BMEI1261 in contig AE008917_GR.
KEGG	bme:BMEI1261.
NMPDR	fig|224914.1.peg.1260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YG99.
OMA	CVVGLMH.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1261-MON.
BRENDA	3.4.11.1. 277959.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BRUME

Accession

Primary (citable) accession number: Q8YG99

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)