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Q8YG65 (RSH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase rsh

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
Gene names
Name:rsh
Ordered Locus Names:BMEI1296
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. Ref.2

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Disruption phenotype

Cells show morphological abnormalities such as branching and swelling forms during vegetative growth. It is unable to persist during stationary phase, presumably because of nutrient limitation occurring during this growth phase. It shows an important growth defect in human HeLa cells and in ovine macrophages MOCL3. At four weeks post infection the number of viable bacteria (deletion mutant) in mouse spleen is markedly reduced compared to the wild-type. The deletion mutant shows very low levels or absence of VirB at all time points of growth. Ref.2

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence caution

The sequence AAL52477.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 750750GTP pyrophosphokinase rsh
PRO_0000322561

Regions

Domain45 – 144100HD
Domain676 – 75075ACT

Sequences

Sequence LengthMass (Da)Tools
Q8YG65 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 1BF6850C0FA147C7

FASTA75083,999
        10         20         30         40         50         60 
MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHRSQKRA SGDPYFSHPL EVAAILTDMH 

        70         80         90        100        110        120 
LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 

       130        140        150        160        170        180 
KLLLAISEDV RVLLVKLADR LHNMRTLGVM REDKRLRIAE ETMDIYAPLA GRMGMQDMRE 

       190        200        210        220        230        240 
ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 

       250        260        270        280        290        300 
WSVFRKMESK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 

       310        320        330        340        350        360 
NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 

       370        380        390        400        410        420 
AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 

       430        440        450        460        470        480 
IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 

       490        500        510        520        530        540 
AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSTDVV 

       550        560        570        580        590        600 
KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 

       610        620        630        640        650        660 
KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 

       670        680        690        700        710        720 
VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA NIHNLSMVRT APDFTEMIID 

       730        740        750 
VEVWDLKHLN RIISQLKESA SVSSAKRVNG 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of the facultative intracellular pathogen Brucella melitensis."
DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. expand/collapse author list , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R.
Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 16M / ATCC 23456 / NCTC 10094.
[2]"The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB."
Dozot M., Boigegrain R.-A., Delrue R.-M., Hallez R., Ouahrani-Bettache S., Danese I., Letesson J.-J., De Bolle X., Koehler S.
Cell. Microbiol. 8:1791-1802(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN STRINGENT RESPONSE, DISRUPTION PHENOTYPE.
Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52477.1. Different initiation.
PIRAB3414.
RefSeqNP_540213.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YG65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52477; AAL52477; BMEI1296.
GeneID1197007.
KEGGbme:BMEI1296.
PATRIC17852919. VBIBruMel92729_1451.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000018299.
KOK01139.
OrthoDBEOG6SV551.
ProtClustDBCLSK2754108.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1336-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8YG65.

Entry information

Entry nameRSH_BRUME
AccessionPrimary (citable) accession number: Q8YG65
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: February 19, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names