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Q8YG65

- RSH_BRUME

UniProt

Q8YG65 - RSH_BRUME

Protein

GTP pyrophosphokinase rsh

Gene

rsh

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment.1 Publication

    Catalytic activityi

    ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. GTP binding Source: UniProtKB-KW
    4. GTP diphosphokinase activity Source: UniProtKB-EC
    5. kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. guanosine tetraphosphate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-1336-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP pyrophosphokinase rsh (EC:2.7.6.5)
    Alternative name(s):
    (p)ppGpp synthase
    ATP:GTP 3'-pyrophosphotransferase
    Gene namesi
    Name:rsh
    Ordered Locus Names:BMEI1296
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Pathology & Biotechi

    Disruption phenotypei

    Cells show morphological abnormalities such as branching and swelling forms during vegetative growth. It is unable to persist during stationary phase, presumably because of nutrient limitation occurring during this growth phase. It shows an important growth defect in human HeLa cells and in ovine macrophages MOCL3. At four weeks post infection the number of viable bacteria (deletion mutant) in mouse spleen is markedly reduced compared to the wild-type. The deletion mutant shows very low levels or absence of VirB at all time points of growth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 750750GTP pyrophosphokinase rshPRO_0000322561Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI1296.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YG65.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 144100HDAdd
    BLAST
    Domaini676 – 75075ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 HD domain.Curated

    Phylogenomic databases

    HOGENOMiHOG000018299.
    KOiK01139.
    OrthoDBiEOG6SV551.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YG65-1 [UniParc]FASTAAdd to Basket

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    MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHRSQKRA SGDPYFSHPL    50
    EVAAILTDMH LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL 100
    TKLKKLDLVS KKAVQAENLR KLLLAISEDV RVLLVKLADR LHNMRTLGVM 150
    REDKRLRIAE ETMDIYAPLA GRMGMQDMRE ELEELAFRYI NPDAWRAVTD 200
    RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP WSVFRKMESK 250
    GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 300
    NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP 350
    HKISTETNAY AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR 400
    LIALPRGATP IDFAYAVHTD IGDSCVGAKV NGRIMPLMTE LKNGDEVDII 450
    RSKAQVPPAA WESLVATGKA RAAIRRATRS AVRKQYSGLG MRILERAFER 500
    AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSTDVV KAVYPDYQDT 550
    RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 600
    KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA 650
    YDDQPERWID VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA 700
    NIHNLSMVRT APDFTEMIID VEVWDLKHLN RIISQLKESA SVSSAKRVNG 750
    Length:750
    Mass (Da):83,999
    Last modified:February 26, 2008 - v2
    Checksum:i1BF6850C0FA147C7
    GO

    Sequence cautioni

    The sequence AAL52477.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52477.1. Different initiation.
    PIRiAB3414.
    RefSeqiNP_540213.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52477; AAL52477; BMEI1296.
    GeneIDi1197007.
    KEGGibme:BMEI1296.
    PATRICi17852919. VBIBruMel92729_1451.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52477.1 . Different initiation.
    PIRi AB3414.
    RefSeqi NP_540213.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YG65.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52477 ; AAL52477 ; BMEI1296 .
    GeneIDi 1197007.
    KEGGi bme:BMEI1296.
    PATRICi 17852919. VBIBruMel92729_1451.

    Phylogenomic databases

    HOGENOMi HOG000018299.
    KOi K01139.
    OrthoDBi EOG6SV551.

    Enzyme and pathway databases

    BioCyci BMEL224914:GCJ0-1336-MONOMER.

    Miscellaneous databases

    PROi Q8YG65.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.
    2. "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB."
      Dozot M., Boigegrain R.-A., Delrue R.-M., Hallez R., Ouahrani-Bettache S., Danese I., Letesson J.-J., De Bolle X., Koehler S.
      Cell. Microbiol. 8:1791-1802(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN STRINGENT RESPONSE, DISRUPTION PHENOTYPE.
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiRSH_BRUME
    AccessioniPrimary (citable) accession number: Q8YG65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3