Q8YG35 (GLNE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-ammonia-ligase adenylyltransferase
EC=2.7.7.42

Alternative name(s):
Glutamine-synthetase adenylyltransferase
Short name=ATase
[Glutamate--ammonia-ligase] adenylyltransferase

Gene names

Name:	glnE
Ordered Locus Names:	BMEI1327

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenylation and deadenylation of glutamate--ammonia ligase By similarity. HAMAP-Rule MF_00802
Catalytic activity	ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. HAMAP-Rule MF_00802
Sequence similarities	Belongs to the GlnE family.
Sequence caution	The sequence AAL52508.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [glutamate-ammonia-ligase] adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 983	983	Glutamate-ammonia-ligase adenylyltransferase HAMAP-Rule MF_00802		PRO_0000209235
Regions
	Region	117 – 328	212	GlnE 1 HAMAP-Rule MF_00802
	Region	631 – 847	217	GlnE 2 HAMAP-Rule MF_00802

Sequences

	Sequence		Length	Mass (Da)
	Q8YG35 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 012BD3A12F7F2A9D	FASTA	983	109,444
	10 20 30 40 50 60 MTVENAKALF FERNLCALTP LDPERASAFL ADLEARAREE ELAGVVALLG RKKAADFLSA 70 80 90 100 110 120 ILDLSPFIRE ALTRQPRILD RIVSATPESA LEAILDEISA SGTVAGVSES ELMTSLRQLK 130 140 150 160 170 180 REAHVLIALC DLARIFNTET TTDRLTDLAE ACTGAAVRFL LLDADAAGRI NLPDRSNPEK 190 200 210 220 230 240 DCGWIVLGMG KFGARELNYS SDIDLIVFID ETKPAIGDPY ECVDTFSRLT RRLVRILQDR 250 260 270 280 290 300 TGDGYVFRVD LRLRPDPGST PLAIPVGAAL HYYEGRGQNW ERAAMIKARP VAGDRLSGKQ 310 320 330 340 350 360 ILAELSPYVW RKYLDYAAIA DVHSIKRQIH AHKGHGDIAV RGHNVKLGRG GIREIEFFVQ 370 380 390 400 410 420 TQQLIAGGRF PELRGNQTVP MLARLAERGW ITQQARDALA QEYWFLRDVE HRIQMIADEQ 430 440 450 460 470 480 THILPEDDEG FARVSHMMGY ADPAEFSEIF LAALKVVEKQ YAALFEQAPE LGAASGNLVF 490 500 510 520 530 540 TGDVDDPGTL ETLSAMGYER SSDICRVIRT WHFGRYRATQ SAEARERLTE LTPALLKAFA 550 560 570 580 590 600 ETRRADESLL RFDGFLQGLP AGIQLFSLLQ SNPRLLNLLV MIMSAAPRLA DIITRNPHVF 610 620 630 640 650 660 DGLLDPAIFS EVPTRAYLEE RLRAFLGSAT DFEEVLDRLR IFAAEHRFLI GIRLLTGAIN 670 680 690 700 710 720 GVRAGQAFSD LAELMVGRAL EAVEAELQRR HGKVKGAKVA LLAMGKLGSR ELTAGSDVDL 730 740 750 760 770 780 ILLYDHDKDA EESDGEKPLA PSKYYIRLTQ RLIAALSAPT AEGVLYEVDM RLRPSGNKGP 790 800 810 820 830 840 VATHIEAFGK YQRNDAWTWE HMALTRARPI HGDEAFIARI KVDIEDVLAM PRDVRKLAGD 850 860 870 880 890 900 VREMRELIAQ EKPPRDDWDL KLKPGGIIDL EFIAQFATLA GYVKKTPRPF ATEEVLANLD 910 920 930 940 950 960 PFFADPAMVD GLVEAHRFYT NLSQAIRLCL NDSAGLDQFP PGMRELLCRV AGLPDIERIE 970 980 YELLEHYRLV RAAFDKLVGH GAD

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52508.1. Different initiation.
PIR	AI3417.
RefSeq	NP_540244.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YG35.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1327.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52508; AAL52508; BMEI1327.
GeneID	1197038.
KEGG	bme:BMEI1327.
PATRIC	17852996. VBIBruMel92729_1488.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000256492.
KO	K00982.
OMA	DQGRDWE.
ProtClustDB	PRK14108.
Family and domain databases
HAMAP	MF_00802. GlnE.
InterPro	IPR023057. GlnE. IPR005190. GlnE_rpt_dom. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
Pfam	PF08335. GlnD_UR_UTase. 2 hits. PF03710. GlnE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNE_BRUME

Accession

Primary (citable) accession number: Q8YG35

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program