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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:BMEI1327
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092351 – 983Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST983

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0886.

Structurei

3D structure databases

ProteinModelPortaliQ8YG35.
SMRiQ8YG35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 468Adenylyl removaseUniRule annotationAdd BLAST468
Regioni473 – 983Adenylyl transferaseUniRule annotationAdd BLAST511

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiFTVQLLQ.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8YG35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVENAKALF FERNLCALTP LDPERASAFL ADLEARAREE ELAGVVALLG
60 70 80 90 100
RKKAADFLSA ILDLSPFIRE ALTRQPRILD RIVSATPESA LEAILDEISA
110 120 130 140 150
SGTVAGVSES ELMTSLRQLK REAHVLIALC DLARIFNTET TTDRLTDLAE
160 170 180 190 200
ACTGAAVRFL LLDADAAGRI NLPDRSNPEK DCGWIVLGMG KFGARELNYS
210 220 230 240 250
SDIDLIVFID ETKPAIGDPY ECVDTFSRLT RRLVRILQDR TGDGYVFRVD
260 270 280 290 300
LRLRPDPGST PLAIPVGAAL HYYEGRGQNW ERAAMIKARP VAGDRLSGKQ
310 320 330 340 350
ILAELSPYVW RKYLDYAAIA DVHSIKRQIH AHKGHGDIAV RGHNVKLGRG
360 370 380 390 400
GIREIEFFVQ TQQLIAGGRF PELRGNQTVP MLARLAERGW ITQQARDALA
410 420 430 440 450
QEYWFLRDVE HRIQMIADEQ THILPEDDEG FARVSHMMGY ADPAEFSEIF
460 470 480 490 500
LAALKVVEKQ YAALFEQAPE LGAASGNLVF TGDVDDPGTL ETLSAMGYER
510 520 530 540 550
SSDICRVIRT WHFGRYRATQ SAEARERLTE LTPALLKAFA ETRRADESLL
560 570 580 590 600
RFDGFLQGLP AGIQLFSLLQ SNPRLLNLLV MIMSAAPRLA DIITRNPHVF
610 620 630 640 650
DGLLDPAIFS EVPTRAYLEE RLRAFLGSAT DFEEVLDRLR IFAAEHRFLI
660 670 680 690 700
GIRLLTGAIN GVRAGQAFSD LAELMVGRAL EAVEAELQRR HGKVKGAKVA
710 720 730 740 750
LLAMGKLGSR ELTAGSDVDL ILLYDHDKDA EESDGEKPLA PSKYYIRLTQ
760 770 780 790 800
RLIAALSAPT AEGVLYEVDM RLRPSGNKGP VATHIEAFGK YQRNDAWTWE
810 820 830 840 850
HMALTRARPI HGDEAFIARI KVDIEDVLAM PRDVRKLAGD VREMRELIAQ
860 870 880 890 900
EKPPRDDWDL KLKPGGIIDL EFIAQFATLA GYVKKTPRPF ATEEVLANLD
910 920 930 940 950
PFFADPAMVD GLVEAHRFYT NLSQAIRLCL NDSAGLDQFP PGMRELLCRV
960 970 980
AGLPDIERIE YELLEHYRLV RAAFDKLVGH GAD
Length:983
Mass (Da):109,444
Last modified:January 4, 2005 - v2
Checksum:i012BD3A12F7F2A9D
GO

Sequence cautioni

The sequence AAL52508 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52508.1. Different initiation.
PIRiAI3417.
RefSeqiWP_004683327.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL52508; AAL52508; BMEI1327.
GeneIDi29594178.
KEGGibme:BMEI1327.
bmel:DK63_76.
PATRICifig|224914.52.peg.79.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52508.1. Different initiation.
PIRiAI3417.
RefSeqiWP_004683327.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliQ8YG35.
SMRiQ8YG35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_0886.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52508; AAL52508; BMEI1327.
GeneIDi29594178.
KEGGibme:BMEI1327.
bmel:DK63_76.
PATRICifig|224914.52.peg.79.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiFTVQLLQ.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_BRUME
AccessioniPrimary (citable) accession number: Q8YG35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: June 7, 2017
This is version 81 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.