Reviewed, UniProtKB/Swiss-Prot Q8YFY2 (BETA_BRUME)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	BMEI1380

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.
Sequence caution	The sequence AAL52561.1 differs from that shown. Reason: Frameshift at position 426.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase HAMAP MF_00750		PRO_0000205584
Regions
	Nucleotide binding	4 – 33	30	FAD Probable

Sequences

	Sequence		Length	Mass (Da)
	Q8YFY2-1 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: FBB2B0956C20252B	FASTA	549	60,665
	10 20 30 40 50 60 MEADFVIIGS GSAGSAMAYR LSEDGRYSVI VIEYGVPDVG PLIQMPAALS FPMNMETYDW 70 80 90 100 110 120 GFSSEPEPHI RGRSLVTPRG KVLGGSSSIN GMVYVRGHAC DFDHWSQSGA RGWAYADVLP 130 140 150 160 170 180 YFKRMENSQG GQEGWRGTNG PLYVQRGKRD NPLFHAFVEA GHQAGFEVTD DYNGEKQEGF 190 200 210 220 230 240 GPMEQTIHNG RRWSAANAYL KPALKRPNVK LVKGFARKIV LEGKRAVGVE IEAGRTFSTI 250 260 270 280 290 300 RARREVIIAA SSINSPKLLM LSGIGPAAHL KEHGIDLVAD RPGVGQNLQD HLEVYIQQEC 310 320 330 340 350 360 TQPITLYSKL NLFSKARIGV EWLLFKTGDG ATNHFESAAF VRSKAGVEYP DIQYHFLPVA 370 380 390 400 410 420 IRYDGKAAAQ SHGFQAHVGP MRSKSRGSVT LRSANPREKP VIKFNYMSHE DDWADFRHCV 430 440 450 460 470 480 RLTREIFGQA AFDPYRGAEI QPGAHVQTDD EIDNFIREHV ESAFHPCGTC KMGAVDDPMA 490 500 510 520 530 540 VVDPECRVIG VEGLRVADSS IFPRITNGNL NGPSIMVGEK ASGHILGRTP LARSNQEPWI NPRWQVSDR

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52561.1. Frameshift.
PIR	AF3424.
RefSeq	NP_540297.1.
3D structure databases
SMR	Q8YFY2. Positions 2-525.
ModBase	Search...
Genome annotation databases
GeneID	1197091.
GenomeReviews	Gene locus BMEI1380 in contig AE008917_GR.
KEGG	bme:BMEI1380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734713.
PhylomeDB	Q8YFY2.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1380-MONOMER.
BRENDA	1.1.99.1. 277959.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BRUME

Accession

Primary (citable) accession number: Q8YFY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	February 9, 2010
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)