Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8YFU1 (HIUH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxyisourate hydrolase

Short name=HIU hydrolase
Short name=HIUHase
EC=3.5.2.17
Gene names
Ordered Locus Names:BMEI1429
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length118 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) By similarity.

Catalytic activity

5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.

Subunit structure

Homotetramer By similarity.

Miscellaneous

HIU hydrolysis also occurs spontaneously, but more slowly.

Sequence similarities

Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.

Sequence caution

The sequence AAL52610.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPurine metabolism
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpurine nucleobase metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

transport

Inferred from electronic annotation. Source: InterPro

   Molecular_functionhydroxyisourate hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 1181185-hydroxyisourate hydrolase
PRO_0000050609

Sites

Binding site71Substrate By similarity
Binding site461Substrate By similarity
Binding site1151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YFU1 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: 78CF15B3E0178A8B

FASTA11813,033
        10         20         30         40         50         60 
MGKLSTHVLD TAHGTPAAAM RVELYRIAAS GTPELLKRVV TNLDGRTDAP LLSGDEMRTG 

        70         80         90        100        110 
IYELQFHVAE YFEGRGAELA HEPFLDLIPI RFGIADEDGN YHVPLLVSPW SYSTYRGS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52610.1. Different initiation.
PIRAG3430.
RefSeqNP_540346.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YFU1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1429.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52610; AAL52610; BMEI1429.
GeneID1197140.
KEGGbme:BMEI1429.
PATRIC17853210. VBIBruMel92729_1594.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000251776.
KOK07127.
OMAYRHYSIA.
OrthoDBEOG6038XF.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1471-MONOMER.

Family and domain databases

Gene3D2.60.40.180. 1 hit.
InterProIPR014306. Hydroxyisourate_hydrolase.
IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
IPR023419. Transthyretin_CS.
[Graphical view]
PfamPF00576. Transthyretin. 1 hit.
[Graphical view]
PRINTSPR00189. TRNSTHYRETIN.
SUPFAMSSF49472. SSF49472. 1 hit.
TIGRFAMsTIGR02962. hdxy_isourate. 1 hit.
PROSITEPS00768. TRANSTHYRETIN_1. 1 hit.
PS00769. TRANSTHYRETIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHIUH_BRUME
AccessionPrimary (citable) accession number: Q8YFU1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 7, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names