Q8YFU1 (HIUH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxyisourate hydrolase
Short name=HIU hydrolase
Short name=HIUHase
EC=3.5.2.17

Gene names

Ordered Locus Names:

BMEI1429

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) By similarity.
Catalytic activity	5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.
Subunit structure	Homotetramer By similarity.
Miscellaneous	HIU hydrolysis also occurs spontaneously, but more slowly.
Sequence similarities	Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.
Sequence caution	The sequence AAL52610.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyisourate hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	5-hydroxyisourate hydrolase		PRO_0000050609
Sites
	Binding site	7	1	Substrate By similarity
	Binding site	46	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YFU1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 78CF15B3E0178A8B	FASTA	118	13,033
	10 20 30 40 50 60 MGKLSTHVLD TAHGTPAAAM RVELYRIAAS GTPELLKRVV TNLDGRTDAP LLSGDEMRTG 70 80 90 100 110 IYELQFHVAE YFEGRGAELA HEPFLDLIPI RFGIADEDGN YHVPLLVSPW SYSTYRGS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52610.1. Different initiation.
PIR	AG3430.
RefSeq	NP_540346.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YFU1.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52610; AAL52610; BMEI1429.
GeneID	1197140.
KEGG	bme:BMEI1429.
PATRIC	17853210. VBIBruMel92729_1594.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000251776.
KO	K07127.
OMA	WQATGMA.
ProtClustDB	CLSK898431.
Family and domain databases
Gene3D	2.60.40.180. 1 hit.
InterPro	IPR014306. Hydroxyisourate_hydrolase. IPR023418. Thyroxine_BS. IPR000895. Transthyretin/HIU_hydrolase. IPR023416. Transthyretin/HIU_hydrolase_SF. IPR023419. Transthyretin_CS. [Graphical view]
Pfam	PF00576. Transthyretin. 1 hit. [Graphical view]
PRINTS	PR00189. TRNSTHYRETIN.
SUPFAM	SSF49472. Transthyretin. 1 hit.
TIGRFAMs	TIGR02962. hdxy_isourate. 1 hit.
PROSITE	PS00768. TRANSTHYRETIN_1. 1 hit. PS00769. TRANSTHYRETIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIUH_BRUME

Accession

Primary (citable) accession number: Q8YFU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	November 7, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program