Q8YFS6 (MTB1_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BabI
Short name=M.BabI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog

Gene names

Name:	ccrM
Ordered Locus Names:	BMEI1444

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Modification methylase BabI		PRO_0000363193

Sequences

	Sequence		Length	Mass (Da)
	Q8YFS6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 3ABB8BE232CB72F9	FASTA	403	45,065
	10 20 30 40 50 60 MALTAYLPYA VTIGTSFLRS QVIEYPMSLV RLAHELPIEA PRTAWLDSII KGDCVSALER 70 80 90 100 110 120 LSDHSVDVIF ADPPYNLQLG GDLHRPDQSM VSAVDDHWDQ FESFQAYDAF TRAWLLACRR 130 140 150 160 170 180 VLKPNGTIWV IGSYHNIFRV GTQLQDLGFW LLNDIVWRKT NPMPNFRGRR FQNAHETLIW 190 200 210 220 230 240 ASRDQKGKGY TFNYEAMKAA NDDVQMRSDW LFPICTGSER LKDENGDKVH PTQKPEALLA 250 260 270 280 290 300 RIMMASSKPG DVILDPFFGS GTTGAVAKRL GRHFVGIERE QPYIDAATAR INAVEPLGKA 310 320 330 340 350 360 ELTVMTGKRA EPRVAFTSVM EAGLLRPGTV LCDERRRFAA IVRADGTLTA NGEAGSIHRI 370 380 390 400 GARVQGFDAC NGWTFWHFEE NGVLKPIDAL RKIIREQMAA AGA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52625.1.
PIR	AF3432.
RefSeq	NP_540361.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YFS6.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1444.
Protein family/group databases
REBASE	5746. M.BmeLORF1444P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52625; AAL52625; BMEI1444.
GeneID	1197155.
KEGG	bme:BMEI1444.
PATRIC	17853242. VBIBruMel92729_1610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000125329.
KO	K13581.
OMA	KVHSTQK.
ProtClustDB	CLSK2518600.
Enzyme and pathway databases
BioCyc	BMEL224914:GCJ0-1486-MONOMER.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BRUME

Accession

Primary (citable) accession number: Q8YFS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program