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Q8YFK1

- PUR2_BRUME

UniProt

Q8YFK1 - PUR2_BRUME

Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi282 – 2821Magnesium or manganeseUniRule annotation
    Metal bindingi284 – 2841Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi133 – 19361ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. manganese ion binding Source: UniProtKB-HAMAP
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
    Alternative name(s):
    GARSUniRule annotation
    Glycinamide ribonucleotide synthetaseUniRule annotation
    Phosphoribosylglycinamide synthetaseUniRule annotation
    Gene namesi
    Name:purDUniRule annotation
    Ordered Locus Names:BMEI1519
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Phosphoribosylamine--glycine ligasePRO_0000151437Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI1519.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YFK1.
    SMRiQ8YFK1. Positions 1-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 312206ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GARS family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000033463.
    KOiK01945.
    OMAiIMMRLKT.
    OrthoDBiEOG69SKD1.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YFK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVLLIGSGG REHALAWKLA ASPLLEKLYC ALGNPGIAAV AELADIGVDD    50
    HAALIAFAKE KHIDLVVVGP EAPLVAGLAD EMRAEGIRVF GPSRAAAQLE 100
    GSKGFTKDLC ARFNIPTGAY GRFNNAPKAK AYIRQQGAPI VVKADGLAAG 150
    KGVVVAMTLQ EALDAVDSCF EGAFGAAGAE VVVEEFLDGE EASFFCICDG 200
    KTALPLGSAQ DHKRVGDGDT GPNTGGMGAY APAPVMTPEM VARTMRELIE 250
    PTMRGMAEIG APFSGILFAG LMITSDGPKL IEYNTRFGDP ECQVLMMRLN 300
    SDLLALVNAA VDGRLDEVSL EWKDEPALTV VMAAEGYPAN VKKGSVIRDL 350
    EKLESIDGVK LFHAGTALKD GAIVASGGRV LNITATAATV AQAQARAYEA 400
    LKLIDWPEGF YRSDIGWRAV EREKANR 427
    Length:427
    Mass (Da):44,973
    Last modified:March 27, 2002 - v1
    Checksum:iC4ADF63267DD5826
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52700.1.
    PIRiAI3441.
    RefSeqiNP_540436.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52700; AAL52700; BMEI1519.
    GeneIDi1197230.
    KEGGibme:BMEI1519.
    PATRICi17796919. VBIBruMel146950_0279.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52700.1 .
    PIRi AI3441.
    RefSeqi NP_540436.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YFK1.
    SMRi Q8YFK1. Positions 1-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1519.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52700 ; AAL52700 ; BMEI1519 .
    GeneIDi 1197230.
    KEGGi bme:BMEI1519.
    PATRICi 17796919. VBIBruMel146950_0279.

    Phylogenomic databases

    HOGENOMi HOG000033463.
    KOi K01945.
    OMAi IMMRLKT.
    OrthoDBi EOG69SKD1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .

    Miscellaneous databases

    PROi Q8YFK1.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiPUR2_BRUME
    AccessioniPrimary (citable) accession number: Q8YFK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3