Reviewed, UniProtKB/Swiss-Prot Q8YFK1 (PUR2_BRUME)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	BMEI1519

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151437
Regions
	Domain	107 – 312	206	ATP-grasp
	Nucleotide binding	133 – 193	61	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YFK1-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: C4ADF63267DD5826	FASTA	427	44,973
	10 20 30 40 50 60 MKVLLIGSGG REHALAWKLA ASPLLEKLYC ALGNPGIAAV AELADIGVDD HAALIAFAKE 70 80 90 100 110 120 KHIDLVVVGP EAPLVAGLAD EMRAEGIRVF GPSRAAAQLE GSKGFTKDLC ARFNIPTGAY 130 140 150 160 170 180 GRFNNAPKAK AYIRQQGAPI VVKADGLAAG KGVVVAMTLQ EALDAVDSCF EGAFGAAGAE 190 200 210 220 230 240 VVVEEFLDGE EASFFCICDG KTALPLGSAQ DHKRVGDGDT GPNTGGMGAY APAPVMTPEM 250 260 270 280 290 300 VARTMRELIE PTMRGMAEIG APFSGILFAG LMITSDGPKL IEYNTRFGDP ECQVLMMRLN 310 320 330 340 350 360 SDLLALVNAA VDGRLDEVSL EWKDEPALTV VMAAEGYPAN VKKGSVIRDL EKLESIDGVK 370 380 390 400 410 420 LFHAGTALKD GAIVASGGRV LNITATAATV AQAQARAYEA LKLIDWPEGF YRSDIGWRAV EREKANR

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL52700.1.
PIR	AI3441.
RefSeq	NP_540436.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1197230.
GenomeReviews	Gene locus BMEI1519 in contig AE008917_GR.
KEGG	bme:BMEI1519.
NMPDR	fig|224914.1.peg.1518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YFK1.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1519-MON.
BRENDA	6.3.4.13. 277959.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_BRUME

Accession

Primary (citable) accession number: Q8YFK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)