Q8YFJ0 (SYGA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	BMEI1530

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAL52711.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_0000072828

Sequences

	Sequence		Length	Mass (Da)
	Q8YFJ0 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 6DD60DEDF2F376A7	FASTA	308	34,945
	10 20 30 40 50 60 MHPTRSFQGL ILTLHNYWAE HGCAILQPYD MEVGAGTFHP ATTLRSLGPK PWKAAYVQPS 70 80 90 100 110 120 RRPKDGRYGE NPNRLQHYYQ YQVLIKPSPP NLQDLYLGSL KAIGLDPTLH DVRFVEDDWE 130 140 150 160 170 180 SPTLGAWGLG WECWCDGMEV SQFTYFQQVC GIECSPVAGE LTYGLERLAM YVQGVDNVYD 190 200 210 220 230 240 LNFNGLEGDE KVTYGDVFLQ AEQEYSRYNF EMANTETLHQ HFIDAERECE AILKAGSTGE 250 260 270 280 290 300 NSLHKCVFPA YDQCIKASHV FNLMDARGVI SVTERQGYIL RVRNLARQCG EAFLLTDAGG FNFKREGE

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52711.1. Different initiation.
PIR	AD3443.
RefSeq	NP_540447.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YFJ0.
SMR	Q8YFJ0. Positions 6-297.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1197241.
GenomeReviews	Gene locus BMEI1530 in contig AE008917_GR.
KEGG	bme:BMEI1530.
PATRIC	17853412. VBIBruMel92729_1696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	Q8YFJ0.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1530-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_BRUME

Accession

Primary (citable) accession number: Q8YFJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	April 30, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program