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Q8YFI3

- ISPE_BRUME

UniProt

Q8YFI3 - ISPE_BRUME

Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Mar 2002)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

    Catalytic activityi

    ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei18 – 181UniRule annotation
    Active sitei146 – 1461UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 11411ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
    2. terpenoid biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Isoprene biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00056; UER00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
    Short name:
    CMKUniRule annotation
    Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
    Gene namesi
    Name:ispEUniRule annotation
    Ordered Locus Names:BMEI1537
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2992994-diphosphocytidyl-2-C-methyl-D-erythritol kinasePRO_0000189195Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI1537.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YFI3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000019600.
    KOiK00919.
    OMAiEGYHPIS.
    OrthoDBiEOG62VNQ2.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00061. IspE.
    InterProiIPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
    PfamiPF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF010376. IspE. 1 hit.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00154. ispE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8YFI3-1 [UniParc]FASTAAdd to Basket

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    MTAFQNAGPS ITRLAPAKIN LALHVTGRRD DGYHLLDMLV VFADQGDRIH    50
    IEKAGSDSFT VSGPFASGIP AGRGNLVLKA RDALRQHGGP DLSPVAIHLE 100
    KNLPIASGIG GGSSDAAATL LALNTLWQLD LDFEMLAAIG LSLGADLPMC 150
    LHGAAHGTPL IARGIGEELN DVSGIAALPM LLVNDGTALA TPDVFRALTR 200
    RENAPLPPPA CEGTDALCAY LRETRNDLLP AAISLAPQIE PKLALLRAKG 250
    ALYARMSGSG ATCFAIFSDK SALTRAAKEI ADENPGWFAV PSHSFPSRA 299
    Length:299
    Mass (Da):31,207
    Last modified:March 5, 2002 - v1
    Checksum:iA9B887F91686DCFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52718.1.
    PIRiAC3444.
    RefSeqiNP_540454.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52718; AAL52718; BMEI1537.
    GeneIDi1197248.
    KEGGibme:BMEI1537.
    PATRICi17796883. VBIBruMel146950_0261.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52718.1 .
    PIRi AC3444.
    RefSeqi NP_540454.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YFI3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1537.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52718 ; AAL52718 ; BMEI1537 .
    GeneIDi 1197248.
    KEGGi bme:BMEI1537.
    PATRICi 17796883. VBIBruMel146950_0261.

    Phylogenomic databases

    HOGENOMi HOG000019600.
    KOi K00919.
    OMAi EGYHPIS.
    OrthoDBi EOG62VNQ2.

    Enzyme and pathway databases

    UniPathwayi UPA00056 ; UER00094 .

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPi MF_00061. IspE.
    InterProi IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR004424. IspE.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR20861:SF2. PTHR20861:SF2. 1 hit.
    Pfami PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF010376. IspE. 1 hit.
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsi TIGR00154. ispE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiISPE_BRUME
    AccessioniPrimary (citable) accession number: Q8YFI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3