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Q8YFI3 (ISPE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:BMEI1537
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2992994-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_0000189195

Regions

Nucleotide binding104 – 11411ATP Potential

Sites

Active site181 By similarity
Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YFI3 [UniParc].

Last modified March 5, 2002. Version 1.
Checksum: A9B887F91686DCFF

FASTA29931,207
        10         20         30         40         50         60 
MTAFQNAGPS ITRLAPAKIN LALHVTGRRD DGYHLLDMLV VFADQGDRIH IEKAGSDSFT 

        70         80         90        100        110        120 
VSGPFASGIP AGRGNLVLKA RDALRQHGGP DLSPVAIHLE KNLPIASGIG GGSSDAAATL 

       130        140        150        160        170        180 
LALNTLWQLD LDFEMLAAIG LSLGADLPMC LHGAAHGTPL IARGIGEELN DVSGIAALPM 

       190        200        210        220        230        240 
LLVNDGTALA TPDVFRALTR RENAPLPPPA CEGTDALCAY LRETRNDLLP AAISLAPQIE 

       250        260        270        280        290 
PKLALLRAKG ALYARMSGSG ATCFAIFSDK SALTRAAKEI ADENPGWFAV PSHSFPSRA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52718.1.
PIRAC3444.
RefSeqNP_540454.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YFI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1537.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52718; AAL52718; BMEI1537.
GeneID1197248.
KEGGbme:BMEI1537.
PATRIC17796883. VBIBruMel146950_0261.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000019600.
KOK00919.
OMAFADCADR.
OrthoDBEOG62VNQ2.
ProtClustDBPRK14608.

Enzyme and pathway databases

UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_BRUME
AccessionPrimary (citable) accession number: Q8YFI3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names