Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18UniRule annotation1
Active sitei146UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 114ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEL224914:G1FZL-1650-MONOMER
UniPathwayiUPA00056; UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:BMEI1537
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001891951 – 2994-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST299

Proteomic databases

PRIDEiQ8YFI3

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0688

Structurei

3D structure databases

ProteinModelPortaliQ8YFI3
SMRiQ8YFI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTR Bacteria
COG1947 LUCA
HOGENOMiHOG000019600
KOiK00919
OMAiRWPSPAK

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YFI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFQNAGPS ITRLAPAKIN LALHVTGRRD DGYHLLDMLV VFADQGDRIH
60 70 80 90 100
IEKAGSDSFT VSGPFASGIP AGRGNLVLKA RDALRQHGGP DLSPVAIHLE
110 120 130 140 150
KNLPIASGIG GGSSDAAATL LALNTLWQLD LDFEMLAAIG LSLGADLPMC
160 170 180 190 200
LHGAAHGTPL IARGIGEELN DVSGIAALPM LLVNDGTALA TPDVFRALTR
210 220 230 240 250
RENAPLPPPA CEGTDALCAY LRETRNDLLP AAISLAPQIE PKLALLRAKG
260 270 280 290
ALYARMSGSG ATCFAIFSDK SALTRAAKEI ADENPGWFAV PSHSFPSRA
Length:299
Mass (Da):31,207
Last modified:March 5, 2002 - v1
Checksum:iA9B887F91686DCFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL52718.1
PIRiAC3444
RefSeqiWP_004683026.1, NZ_GG703778.1

Genome annotation databases

EnsemblBacteriaiAAL52718; AAL52718; BMEI1537
GeneIDi29594382
KEGGibme:BMEI1537
bmel:DK63_1955
PATRICifig|224914.52.peg.2056

Similar proteinsi

Entry informationi

Entry nameiISPE_BRUME
AccessioniPrimary (citable) accession number: Q8YFI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health