Q8YFH6 (ATP6_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit a

Alternative name(s):
ATP synthase F0 sector subunit a
F-ATPase subunit 6

Gene names

Name:	atpB
Ordered Locus Names:	BMEI1546

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane By similarity. HAMAP-Rule MF_01393
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01393.
Sequence similarities	Belongs to the ATPase A chain family.
Sequence caution	The sequence AAC08028.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL52727.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell proton-transporting ATP synthase complex, coupling factor F(o) Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	proton-transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	ATP synthase subunit a HAMAP-Rule MF_01393		PRO_0000362256
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Transmembrane	185 – 205	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
Natural variations
	Natural variant	171	1	S → T in strain 133.
	Natural variant	189 – 191	3	VFA → FSP in strain 133.

Sequences

	Sequence		Length	Mass (Da)
	Q8YFH6 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 0322177F778BAE6D	FASTA	249	26,979
	10 20 30 40 50 60 MANDPIHQFQ VSRWIPIDVG GVDLSFTNVS AFMVATVVLA SGFLYLTSSG RGLIPTRLQS 70 80 90 100 110 120 VSEMAYEFVA TSLRDSAGSK GMKFFPFVFS LFMFVLVANF IGLFPYFYTV TSQIIVTFAL 130 140 150 160 170 180 SLLVIGTVIF YGFFKHGFGF LKLFVPSGVP GIIVPLVVLI EIISFLSRPI SLSVRLFANM 190 200 210 220 230 240 LAGHITLKVF AGFVVSLSSL GALGIGGAVL PLLMTVAITA LEFLVAFLQA YVFTVLTCMY INDAVHPGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"Identification of four genes of the Brucella melitensis ATP synthase operon F0 sector: relationship with the Rhodospirillaceae family." Hernandez-Castro R., Verdugo-Rodriguez A., Gutierrez-Pabello J.A., Adams L.G., Suarez-Guemes F., Sahagun-Ruiz A. Microb. Comp. Genomics 5:163-171(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 69-249. Strain: 133.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52727.1. Different initiation. AF054609 Genomic DNA. Translation: AAC08028.1. Different initiation.
PIR	AD3445.
RefSeq	NP_540463.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YFH6.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1546.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52727; AAL52727; BMEI1546.
GeneID	1197257.
KEGG	bme:BMEI1546.
PATRIC	17796861. VBIBruMel146950_0250.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000253874.
KO	K02108.
ProtClustDB	PRK05815.
Family and domain databases
Gene3D	1.20.120.220. 1 hit.
HAMAP	MF_01393. ATP_synth_a_bact.
InterPro	IPR000568. ATPase_F0-cplx_asu. IPR023011. ATPase_F0-cplx_asu_AS. [Graphical view]
PANTHER	PTHR11410. PTHR11410. 1 hit.
Pfam	PF00119. ATP-synt_A. 1 hit. [Graphical view]
PRINTS	PR00123. ATPASEA.
SUPFAM	SSF81336. ATPase_F0_A. 1 hit.
TIGRFAMs	TIGR01131. ATP_synt_6_or_A. 1 hit.
PROSITE	PS00449. ATPASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATP6_BRUME

Accession

Primary (citable) accession number: Q8YFH6
Secondary accession number(s): O68817

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program