Q8YF73 (UREF2_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF 2

Gene names

Name:	ureF2
Ordered Locus Names:	BMEI1650

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Urease accessory protein UreF 2 HAMAP MF_01385		PRO_0000344089

Sequences

	Sequence		Length	Mass (Da)
	Q8YF73 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F0D10C2F06ADC49A	FASTA	228	24,446
	10 20 30 40 50 60 MTIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV 70 80 90 100 110 120 RRGSGWNDAV LCAESWRCTM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFLAAARSWP 130 140 150 160 170 180 CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA 190 200 210 220 IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52831.1.
PIR	AD3458.
RefSeq	NP_540567.1. NC_003317.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1197361.
GenomeReviews	Gene locus BMEI1650 in contig AE008917_GR.
KEGG	bme:BMEI1650.
NMPDR	fig|224914.1.peg.1649.
PATRIC	17853682. VBIBruMel92729_1829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK864150.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1650-MONOMER.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF2_BRUME

Accession

Primary (citable) accession number: Q8YF73

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program