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Protein

Urease subunit alpha 1

Gene

ureC1

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi138 – 1381Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi219 – 2191Nickel 1; via carbamate groupUniRule annotation
Metal bindingi219 – 2191Nickel 2; via carbamate groupUniRule annotation
Binding sitei221 – 2211SubstrateUniRule annotation
Metal bindingi248 – 2481Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi274 – 2741Nickel 2; via tele nitrogenUniRule annotation
Active sitei322 – 3221Proton donorUniRule annotation
Metal bindingi362 – 3621Nickel 1UniRule annotation

GO - Molecular functioni

  1. nickel cation binding Source: UniProtKB-HAMAP
  2. urease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alpha 1UniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alpha 1UniRule annotation
Gene namesi
Name:ureC1UniRule annotation
Ordered Locus Names:BMEI1652
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Urease subunit alpha 1PRO_0000234143Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BMEI1652.

Structurei

3D structure databases

ProteinModelPortaliQ8YF72.
SMRiQ8YF72. Positions 3-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 570440UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000075064.
KOiK01428.
OMAiIVDHWGI.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro_1.
IPR011059. Metal-dep_hydrolase_composite.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YF72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARISRATY AQMFGPTVGD KVRLADTDLI IEVERDLTTY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ LSRAEGAMDT VITNALILDH SGIYKADIGL LDGRIALIGK
110 120 130 140 150
AGNPDTQPGI SIIIGPGTEI IAGEGKIVTA GGIDTHVHFI SPQQVDEALN
160 170 180 190 200
AGITCMVGGG TGPAHGTLAT TCTPGPWHIA RLIQSFDGLP MNIGVFGKGN
210 220 230 240 250
ASLPGALEEM VRAGACGLKL HEDWGCTPAA IDNCLSVADH FDVQVAIHTD
260 270 280 290 300
TLNEGGFVED TLNAFKGRTI HSFHTEGAGG GHAPDIIRVC QYPNVLPAST
310 320 330 340 350
NPTRPYTVNT IAEHLDMLMV CHHLSPAIPE DIAFAESRIR KETIAAEDIL
360 370 380 390 400
HDMGAFSIIS SDSQAMGRVG EMIIRCWQTA DKMKKQRGSL PDDRPGNDNY
410 420 430 440 450
RARRYIAKYT INPAIAHGMA HEIGSVEVGK RADLVLWNSA FFGVKPDMVL
460 470 480 490 500
LGGWIATAPM GDANGSIPTP QPMHTRPMFG SFGKALTNTS ITFVSQAAMD
510 520 530 540 550
EGLREKIGVD KQLVAVVNTR GGIGKHSMIL NNAMPQMEVD PETYEVRADG
560 570
ELLTCEPVDV VPMAQRYFLF
Length:570
Mass (Da):61,001
Last modified:February 28, 2002 - v1
Checksum:i6487ABAC61D967EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52833.1.
PIRiAF3458.
RefSeqiNP_540569.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52833; AAL52833; BMEI1652.
KEGGibme:BMEI1652.
PATRICi17796614. VBIBruMel146950_0128.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52833.1.
PIRiAF3458.
RefSeqiNP_540569.1. NC_003317.1.

3D structure databases

ProteinModelPortaliQ8YF72.
SMRiQ8YF72. Positions 3-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BMEI1652.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52833; AAL52833; BMEI1652.
KEGGibme:BMEI1652.
PATRICi17796614. VBIBruMel146950_0128.

Phylogenomic databases

HOGENOMiHOG000075064.
KOiK01428.
OMAiIVDHWGI.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro_1.
IPR011059. Metal-dep_hydrolase_composite.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiURE11_BRUME
AccessioniPrimary (citable) accession number: Q8YF72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2006
Last sequence update: February 28, 2002
Last modified: March 31, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.